大肠杆菌胞质外区室中响应蛋白质错误折叠的信号转导途径：两种新型磷酸蛋白磷酸酶PrpA和PrpB的作用
Signal transduction pathways in response to protein misfolding in the extracytoplasmic compartments of E. coli: role of two new phosphoprotein phosphatases PrpA and PrpB.
作者信息
Missiakas D, Raina S
机构信息
Centre Medical Universitaire, Departement de Biochimie Medicale, Geneve, Switzerland.
出版信息
EMBO J. 1997 Apr 1;16(7):1670-85. doi: 10.1093/emboj/16.7.1670.
Abstract
It is now well established that the sigmaE regulon of Escherichia coli is induced by misfolding of proteins in the periplasm and the outer membrane. htrA belongs to this regulon and encodes a periplasmic protease involved in the degradation of misfolded proteins. htrA transcription is also under the positive control of a two component signal transduction system CpxR CpxA. Closer examination of the putative signal transduction pathway modulating htrA transcription has led us to the identification of two new genes. Biochemical and genetic evidence shows that these two genes encode two phosphoprotein phosphatases, designated PrpA and PrpB. These are the first examples of typical serine/threonine and tyrosine phosphatases described in E. coli. PrpA and PrpB are involved in signaling protein misfolding via the CpxR CpxA transducing system. In addition, both PrpA and PrpB modulate the phosphorylated status of some other phosphoproteins in E. coli. Finally, we show that PrpA is a heat shock protein.
摘要
现在已经充分证实，大肠杆菌的σE调节子是由周质和外膜中蛋白质的错误折叠所诱导的。htrA属于这个调节子，编码一种参与错误折叠蛋白质降解的周质蛋白酶。htrA转录也受双组分信号转导系统CpxR CpxA的正调控。对调节htrA转录的假定信号转导途径的进一步研究使我们鉴定出两个新基因。生化和遗传学证据表明，这两个基因编码两种磷酸蛋白磷酸酶，分别命名为PrpA和PrpB。这是大肠杆菌中描述的典型丝氨酸/苏氨酸和酪氨酸磷酸酶的首个例子。PrpA和PrpB通过CpxR CpxA转导系统参与信号蛋白错误折叠的信号传递。此外，PrpA和PrpB都调节大肠杆菌中其他一些磷酸蛋白的磷酸化状态。最后，我们表明PrpA是一种热休克蛋白。
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