非戈谢病细胞中人类葡萄糖脑苷脂酶假基因的一种新型转录本。

A novel transcript from a pseudogene for human glucocerebrosidase in non-Gaucher disease cells.

作者信息

Imai K, Nakamura M, Yamada M, Asano A, Yokoyama S, Tsuji S, Ginns E I

机构信息

Department of Biochemistry, Shimane Medical University, Izumo, Japan.

出版信息

Gene. 1993 Dec 22;136(1-2):365-8. doi: 10.1016/0378-1119(93)90497-q.

DOI:10.1016/0378-1119(93)90497-q

PMID:8294033

Abstract

Human glucocerebrosidase (GC)-encoding cDNA clones were isolated from a promyelocytic HL-60 cDNA library and analyzed. A novel cDNA clone was found to originate from a gene referred to as a GC pseudogene. Using the polymerase chain reaction (PCR) with primers specific for the GC pseudogene, we found that all the human cell lines examined, HL-60, K-562, WI-38, HepG2 and HeLa, expressed a pseudogene transcript. In vitro translation of RNA synthesized by transcription of the pseudogene cDNA produced a polypeptide of approximately 30 kDa.

摘要

从早幼粒细胞HL-60 cDNA文库中分离并分析了编码人葡萄糖脑苷脂酶（GC）的cDNA克隆。发现一个新的cDNA克隆源自一个被称为GC假基因的基因。使用针对GC假基因的特异性引物进行聚合酶链反应（PCR），我们发现所有检测的人细胞系，HL-60、K-562、WI-38、HepG2和HeLa，都表达一种假基因转录本。通过假基因cDNA转录合成的RNA的体外翻译产生了一种约30 kDa的多肽。