用于直接克隆PCR产物的新型载体。
New vectors for direct cloning of PCR products.
作者信息
Cha J, Bishai W, Chandrasegaran S
机构信息
Department of Environmental Health Sciences, School of Hygiene and Public Health, Johns Hopkins University, Baltimore, MD 21205.
出版信息
Gene. 1993 Dec 22;136(1-2):369-70. doi: 10.1016/0378-1119(93)90498-r.
We describe the construction of two new vectors for direct cloning of polymerase chain reaction (PCR) products. This was done by inserting a synthetic DNA fragment containing two adjacent XcmI sites between the Asp718 and BamHI sites of the M13mp18 and M13mp19 phages. Cleavage of these M13 derivatives with XcmI will result in a linearized vector with a single thymidine nucleotide at the 3' ends. Thus, these vectors would be very useful for direct cloning of PCR-generated products with high efficiency.
我们描述了两种用于直接克隆聚合酶链反应（PCR）产物的新载体的构建方法。具体做法是在M13mp18和M13mp19噬菌体的Asp718和BamHI位点之间插入一个含有两个相邻XcmI位点的合成DNA片段。用XcmI切割这些M13衍生物会产生一种在3'端带有单个胸腺嘧啶核苷酸的线性化载体。因此，这些载体对于高效直接克隆PCR产生的产物将非常有用。
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