Koller R, Frey M, Liegl C, Meier U, Neumayer C, Gruber H, Meyer V E
Abteilung für Plastische und Rekonstruktive Chirurgie, II. Chirurgischen Universitätsklinik Wien.
Handchir Mikrochir Plast Chir. 1993 Nov;25(6):300-7.
The present study was undertaken to investigate whether the number of myelinated fibers in a sensory nerve used as an autograft influences the result of nerve fiber regeneration in the graft. In 30 rabbits, the left saphenous nerve was used as an autograft. It was coapted to the motor branch to the rectus femoris muscle. Biopsies from both ends of the graft were taken. Animals were separated into four investigation groups, which were reexamined 3, 6, 9 and 12 months after the grafting procedure. Biopsies for histological and morphometric examination were again taken from the proximal and the distal part of the grafts. Thus, the number of myelinated nerve fibers in the different specimens could be obtained by computer-assisted image analysis. At the proximal end of the original saphenous nerve, the number of fibers varied between 1032 and 3100 (mean 2145). Corresponding data obtained from the distal end ranged from 1044 to 3084 myelinated nerve fibers (mean 1724). Fiber numbers in the proximal and distal parts of the grafts before and after regeneration were correlated to each other. In the whole population of specimens (correlation coefficient +/-0) as well as in the experimental subgroups, the quantitative success of neurotization in the grafts did not depend to a high degree upon the fiber numbers in the original saphenous nerve. The correlation coefficient for the proximal part of the graft exceeded 0.5 to only three and twelve months after grafting, but did not reach a level of statistical significance. In the distal part of the graft, the coefficient for a linear correlation was always less than 0.45.
本研究旨在调查用作自体移植的感觉神经中髓鞘化纤维的数量是否会影响移植中神经纤维再生的结果。在30只兔子中，左隐神经用作自体移植。它与股直肌的运动支进行端端吻合。从移植体的两端取活检组织。将动物分为四个研究组，在移植手术后3、6、9和12个月进行复查。再次从移植体的近端和远端取活检组织进行组织学和形态学检查。因此，通过计算机辅助图像分析可以获得不同标本中髓鞘化神经纤维的数量。在原来隐神经的近端，纤维数量在1032至3100之间（平均2145）。从远端获得的相应数据范围为1044至3084条髓鞘化神经纤维（平均1724）。再生前后移植体近端和远端的纤维数量相互关联。在整个标本群体（相关系数±0）以及实验亚组中，移植体中神经化的定量成功在很大程度上并不取决于原来隐神经中的纤维数量。移植体近端的相关系数仅在移植后3个月和12个月超过0.5，但未达到统计学显著水平。在移植体远端，线性相关系数始终小于0.45。
