[Effect of transplant length on functional and morphologic outcome of nerve transplantation--an experimental study].

BACKGROUND

The present study was done in order to clarify whether the sometimes poor results after the use of long grafts for nerve reconstruction are due to the length of the graft itself or due to the concomitant big defect in the soft tissues necessitating the use of long grafts.

METHODS

In 22 rabbits, the saphenous nerve was used as a nerve graft. Animals were separated into three groups with different lengths of the grafts, namely 3 cm (group 1), 5 cm (group 2) and 7 cm (group 3). In one hindlimb, the proximal end of the graft was coapted to the motor nerve branch of vastus medialis. In a second step, the distal end of the graft was coapted to the nerve branch of rectus femoris. After a total period of 15 months the maximum tetanic tensions in the reinnervated rectus femoris and in the contralateral untreated muscle were determined. Biopsies of the graft and the motor branch distal to the graft were taken in order to count the number of regenerated myelinated nerve fibers.

RESULTS

The average maximum tetanic tension in the rectus femoris muscle reinnervated by the 3 cm long graft was 27.2 N; in group 2 the force amounted to 20.4 N. In group 3, the maximum force was 17.6 N, which meant an average loss of 29% compared to the contralateral untreated muscle. In accordance with the functional results, the mean number of regenerated myelinated fibres in the rectus femoris motor branch decreased from 1683 in group 1 to 1136 in group 3.

CONCLUSIONS

The results show that the length of the graft influences the results after nerve grafting to a certain extent, but a combination of other factors like concomitant soft tissue injury and destroyed target organs may also be responsible for some of the poor results after the clinical use of long nerve grafts.