Siemionow M, Romanowski L, Lister G
University of Utah, Division of Plastic and Reconstructive Surgery, Salt Lake City 84132.
J Hand Surg Am. 1993 Nov;18(6):963-71. doi: 10.1016/0363-5023(93)90384-F.
The aim of this study was to determine the effect of leukocyte-endothelial interaction and capillary perfusion on the microcirculation of muscle flaps submitted to ischemia in normothermic and hypothermic conditions. The cremaster muscle flap model was employed. Sixty rats were studied in six groups: normothermic and hypothermic control groups and groups that underwent reperfusion after 4 and 6 hours of ischemia in both normothermic and hypothermic conditions. In each group the following measurements were made: main vessel diameters, red blood cell velocities, the number of perfused capillaries, and the number of leukocytes rolling and sticking in the postcapillary venules. Hypothermia decreased the flow rate significantly and eliminated leukocytes from the microcirculation. Six hours of ischemia under normothermic conditions proved to be lethal for these muscle flaps, whereas hypothermia preserved flap viability.
本研究的目的是确定在正常体温和低温条件下，白细胞与内皮细胞相互作用及毛细血管灌注对遭受缺血的肌皮瓣微循环的影响。采用提睾肌皮瓣模型。60只大鼠被分为六组：正常体温和低温对照组，以及在正常体温和低温条件下缺血4小时和6小时后再灌注的组。在每组中进行以下测量：主要血管直径、红细胞速度、灌注毛细血管数量，以及在毛细血管后微静脉中滚动和黏附的白细胞数量。低温显著降低了流速，并使微循环中无白细胞。在正常体温条件下缺血6小时对这些肌皮瓣是致命的，而低温则保留了皮瓣的活力。
