在梭菌性肌坏死小鼠模型中对抗菌药物与高压氧联合使用的评估。

Evaluation of antimicrobials combined with hyperbaric oxygen in a mouse model of clostridial myonecrosis.

作者信息

Muhvich K H, Anderson L H, Mehm W J

机构信息

Division of Altitude and Hyperbaric Physiology, Armed Forces Institute of Pathology, Washington, DC 20306-6000.

出版信息

J Trauma. 1994 Jan;36(1):7-10. doi: 10.1097/00005373-199401000-00002.

DOI:10.1097/00005373-199401000-00002

PMID:8295252

Abstract

The efficacy of hyperbaric oxygen (HBO) alone and in combination with several antimicrobial agents was evaluated in a lethal model of gas gangrene in mice. Intraperitoneal administration of penicillin, imipenem, clindamycin, or metronidazole immediately followed inoculation of > 10(9) CFU of Clostridium perfringens type A in mice. Mice treated with hyperbaric oxygen were exposed twice a day to 100% oxygen at 303 kilopascals (kPa) pressure for 90 minutes. The total exposure time to HBO for surviving animals was 9 hours. Control (saline-injected) mice treated with HBO alone did not have an enhanced survival rate when compared with mice exposed to air at ambient pressure. Survival of infected mice treated with either clindamycin or metronidazole was significantly longer than that of groups treated with penicillin or imipenem (p < 0.05). Hyperbaric oxygen alone or in combination with the four antimicrobial agents evaluated did not statistically improve survival of mice infected with a lethal dose of C. perfringens.

摘要

在小鼠气性坏疽致死模型中评估了单纯高压氧（HBO）以及高压氧与几种抗菌药物联合使用的疗效。在小鼠接种超过10⁹CFU的A型产气荚膜梭菌后，立即腹腔注射青霉素、亚胺培南、克林霉素或甲硝唑。接受高压氧治疗的小鼠每天两次暴露于303千帕斯卡（kPa）压力的100%氧气中，持续90分钟。存活动物接受高压氧的总暴露时间为9小时。与在常压下暴露于空气中的小鼠相比，单纯接受高压氧治疗的对照（注射生理盐水）小鼠存活率并未提高。接受克林霉素或甲硝唑治疗的感染小鼠的存活时间显著长于接受青霉素或亚胺培南治疗的组（p<0.05）。单纯高压氧或与所评估的四种抗菌药物联合使用，在统计学上均未提高感染致死剂量产气荚膜梭菌小鼠的存活率。