猬迭宫绦虫裂头蚴中花生四烯酸向前列腺素E2的代谢

The metabolism of arachidonic acid to prostaglandin E2 in plerocercoids of Spirometra erinacei.

作者信息

Fukushima T, Isobe A, Hojo N, Shiwaku K, Yamane Y, Torii M

机构信息

Department of Environmental Medicine, Shimane Medical University, Izumo, Japan.

出版信息

Parasitol Res. 1993;79(8):634-8. doi: 10.1007/BF00932504.

DOI:10.1007/BF00932504

PMID:8295899

Abstract

With a simplified method of extracting and purifying prostaglandins, trace prostaglandins (nanogram order) were detected by gas chromatography-mass spectrometry. Arachidonic acid was metabolized to prostaglandin E2 (PGE2) by plerocercoids of Spirometra erinacei, the PGE2 was detected in the medium after incubation with arachidonic acid, and the role of albumin in the absorption of free arachidonic acid by plerocercoids and in the release of its metabolite was investigated. Plerocercoids absorbed arachidonic acid-binding albumin and released PGE2 efficiently. PGE2 is known to suppress the functions of mononuclear cells of the host. The selective release of PGE2 may be related to the escape mechanism of plerocercoids of S. erinacei from the host immune system to become established larva migrans, i.e., sparganosis.

摘要

采用一种简化的前列腺素提取和纯化方法，通过气相色谱 - 质谱法检测到了痕量前列腺素（纳克级）。多房棘球绦虫的裂头蚴可将花生四烯酸代谢为前列腺素E2（PGE2），在与花生四烯酸孵育后的培养基中检测到了PGE2，并研究了白蛋白在裂头蚴吸收游离花生四烯酸及其代谢产物释放过程中的作用。裂头蚴有效吸收了与花生四烯酸结合的白蛋白并释放出PGE2。已知PGE2可抑制宿主单核细胞的功能。PGE2的选择性释放可能与多房棘球绦虫裂头蚴从宿主免疫系统逃逸以形成幼虫移行症（即曼氏裂头蚴病）的机制有关。