Divac I, Ramirez-Gonzalez J A, Rønn L C, Jahnsen H, Regidor J
Department of Medical Physiology, Panum Institute, University of Copenhagen, Denmark.
Neuroreport. 1993 Dec 13;5(3):325-8. doi: 10.1097/00001756-199312000-00037.
We found NADPH-diaphorase (presumably identical with nitric oxide synthase) in pyramidal neurones of the hippocampus in slices that stayed in a chamber for 30 min or longer. In some instances parallel slices showed normal membrane properties when studied electrophysiologically. In freshly made slices the pyramidal neurones were not stained. Thus, after induction of the enzyme, the hippocampal pyramidal neurones can synthesize nitric oxide which may serve as a retrograde messenger in long-term potentiation. The enzyme may also play a role in cell loss seen in slices which stayed in a chamber for 9-22 h before fixation.
我们在置于培养室30分钟或更长时间的脑片海马锥体细胞中发现了NADPH黄递酶（可能与一氧化氮合酶相同）。在某些情况下，并行的脑片在进行电生理研究时显示出正常的膜特性。在新鲜制作的脑片中，锥体细胞未被染色。因此，诱导该酶后，海马锥体细胞可以合成一氧化氮，一氧化氮可能在长时程增强中作为逆行信使发挥作用。该酶也可能在固定前在培养室中放置9 - 22小时的脑片中观察到的细胞损失中起作用。
