Savino W, Santa-Rosa G L
Rev Bras Pesqui Med Biol. 1978 Oct;11(4-5):283-6.
The phenomenon of autofluorescence is reported in the thymus of rabbits; besides reticular epithelial cells, Hassall's bodies also emit autofluorescence. The staining of thymic corpuscle by fluorescein isothiocyanate is also described. This staining is important not only because FITC is commonly used in immunofluorescence and may lead to misinterpretations due to false-positive results, but also for it permits a good precision in the observation of the relationship between Hassall's bodies and reticular epithelial cells. Some hypothesis are set up to explain the autofluorescence, and the fluorochromization of Hassall's bodies by acid fluorochromes.
在兔胸腺中报道了自发荧光现象；除网状上皮细胞外，哈氏小体也发出自发荧光。还描述了异硫氰酸荧光素对胸腺小体的染色。这种染色很重要，不仅因为异硫氰酸荧光素常用于免疫荧光，可能因假阳性结果导致误解，还因为它能在观察哈氏小体与网状上皮细胞之间的关系时提供良好的精确度。提出了一些假设来解释自发荧光以及酸性荧光染料对哈氏小体的荧光染色。
