David L, Nesland J M, Holm R, Sobrinho-Simões M
Institute of Molecular Pathology and Immunology, (IPATIMUP), Medical School, University of Porto, Portugal.
Cancer. 1994 Feb 1;73(3):518-27. doi: 10.1002/1097-0142(19940201)73:3<518::aid-cncr2820730305>3.0.co;2-t.
This study attempted to determine if there is a pattern of matrix/matrix-degrading-enzyme immunoreactivity related to the morphologic types of gastric carcinoma or to their invasiveness.
The authors performed an immunohistochemical study of the basement membrane antigens laminin and collagen IV, fibronectin, and Type IV collagenase in a series of 87 gastric carcinomas and their respective nodal metastases (n = 329).
Laminin expression was observed almost exclusively in carcinomas of the intestinal type. The expression of collagen IV was significantly higher in intestinal (52%) and atypical (44%) carcinomas than in diffuse (10%) carcinomas; collagen IV expression also was significantly correlated with lymphatic invasion and aneuploidy. Ninety percent of the carcinomas expressed fibronectin, mostly in the connective tissue at the invading edge of the tumors; fibronectin expression was significantly related to the expanding growth pattern of the neoplasms. Eighty-six percent of the tumors expressed Type IV collagenase, regardless of the histologic type or invasive properties. No relationship was observed between the expression of any of the antigens and the S-phase fraction of the tumors. No significant differences were found between the immunohistochemical profile of the primary tumors and their metastases.
The authors conclude that the expression of basement membrane antigens is related to the type of gastric carcinomas, rather than the cell differentiation or proliferative activity of the tumors. The putative prognostic meaning of the relationship between collagen IV immunoreactivity and aneuploidy and lymphatic invasiveness of the carcinomas remains to be clarified.
本研究试图确定是否存在与胃癌的形态学类型或其侵袭性相关的基质/基质降解酶免疫反应模式。
作者对87例胃癌及其相应的淋巴结转移灶（n = 329）进行了基底膜抗原层粘连蛋白、IV型胶原、纤连蛋白和IV型胶原酶的免疫组织化学研究。
层粘连蛋白表达几乎仅见于肠型癌。IV型胶原在肠型（52%）和非典型（44%）癌中的表达明显高于弥漫型（10%）癌；IV型胶原表达也与淋巴侵袭和非整倍体显著相关。90%的癌表达纤连蛋白，主要在肿瘤侵袭边缘的结缔组织中；纤连蛋白表达与肿瘤的扩展生长模式显著相关。86%的肿瘤表达IV型胶原酶，无论组织学类型或侵袭特性如何。未观察到任何抗原的表达与肿瘤的S期分数之间的关系。原发肿瘤及其转移灶的免疫组织化学特征之间未发现显著差异。
作者得出结论，基底膜抗原的表达与胃癌的类型有关，而不是与肿瘤的细胞分化或增殖活性有关。IV型胶原免疫反应性与癌的非整倍体和淋巴侵袭之间关系的假定预后意义仍有待阐明。
