Different ouabain sensitivities of Na+/K(+)-ATPase from Poekilocerus bufonius tissues and a possible physiological cost.

1. The properties of Na+/K(+)-transporting ATPase in microsomal preparation from mid-gut of the grasshopper, Poekilocerus bufonius, were investigated and compared with the same enzyme from brain and excretory system. 2. Two components of ATPases activity are present in the three tissues studied. 3. The physiochemical properties of Na+/K(+)-transporting ATPase from mid-gut, brain and excretory system (hind-gut plus Malpighian tubules) are essentially the same. 4. The calculated values of PI50 were 2 (I50 = 1 x 10(-2) M), 3.7 (I50 = 2 x 10(-4) M) and 6.4 (I50 = 3.98 x 10(-7)) for Na+/K(+)-ATPase from mid-gut, excretory system and brain, respectively. The mid-gut contains the most ouabain-resistant Na+/K(+)-ATPase. 5. The results suggest that P. bufonius have developed some tolerance to toxic cardiac glycosides (CGS), but there is a possibility of autotoxicity as indicated by the presence of ouabain-sensitive ATPase from brain tissue. 6. It was concluded that the dissimilarities of Na+/K(+)-ATPases from different tissues of P. bufonius are probably due to tissue-dependent differences in ouabain sensitivity (or isoenzymes pattern) available in the same insect. 7. The atrophy of female flight muscle of P. bufonius suggests the possibility of physiological cost inflicted on insects consuming poisonous plants.