鸡和鲑鱼卵黄中卵黄高磷蛋白的比较研究。

Comparative studies of phosvitin from chicken and salmon egg yolk.

Losso J N, Bogumil R, Nakai S

Department of Food Science, University of British Columbia, Vancouver, BC, Canada.

Comp Biochem Physiol B. 1993 Dec;106(4):919-23. doi: 10.1016/0305-0491(93)90051-6.

Abstract

A method developed for the isolation of phosvitin from chicken egg yolk was successfully applied to the isolation of phosvitin from salmon eggs. 2. Salmon roe phosvitin is smaller in molecular size than chicken egg phosvitin. 3. Circular dichroism spectra of all phosvitins investigated displayed good similarities with spectra showing characteristics of unordered and beta-sheet secondary structure. 4. The main component in the Fourier transform infrared spectra of chicken egg phosvitin is indicative of unordered conformation, whereas the Fourier infrared data of the salmon egg phosvitin are consistent with more of beta-sheet structure compared to the chicken egg phosvitin.

摘要

一种从鸡卵黄中分离卵黄高磷蛋白的方法成功应用于从鲑鱼卵中分离卵黄高磷蛋白。2. 鲑鱼籽卵黄高磷蛋白的分子大小比鸡卵卵黄高磷蛋白小。3. 所有研究的卵黄高磷蛋白的圆二色光谱与显示无序和β-折叠二级结构特征的光谱具有良好的相似性。4. 鸡卵卵黄高磷蛋白的傅里叶变换红外光谱中的主要成分表明其为无序构象，而鲑鱼卵卵黄高磷蛋白的傅里叶红外数据表明，与鸡卵卵黄高磷蛋白相比，其β-折叠结构更多。