Meeusen E, Brandon M
Centre for Animal Biotechnology, School of Veterinary Science, University of Melbourne, Parkville, Victoria, Australia.
Eur J Immunol. 1994 Feb;24(2):469-74. doi: 10.1002/eji.1830240231.
Antibody secreting cell probes (ASC-probes) were generated from the hepatic lymph node (HLN), mesenteric lymph node (MLN) and spleen of rats after infection with the liver fluke, Fasciola hepatica, and used to probe Western blots of parasite antigens. In chronic primary infections, parasite-specific antibodies were only detected in ASC-probes derived from HLN. Seven days after a secondary infection, a restricted response was detected in ASC-probes from the MLN, directed predominantly against an antigen specific to the newly excysted juvenile (NEJ) stage. This NEJ-specific antigen was only recognized by HLN if the second infection was not rejected and the challenge flukes reached the liver. Measurement of the immunoglobulin levels present in the ASC-probes showed significant elevation only in lymph nodes draining sites of recent infection. In addition, when the isotype profiles were determined in ASC-probes derived from different lymph nodes, it was observed that they showed different isotype preferences, in particular IgA for the MLN, IgE for HL.N and IgM for spleen. These results show that discrete and independent immune responses occur in different body compartments of a rat against different stages of a parasite.
抗体分泌细胞探针（ASC探针）是从感染肝吸虫（肝片吸虫）的大鼠的肝淋巴结（HLN）、肠系膜淋巴结（MLN）和脾脏中制备的，并用于检测寄生虫抗原的蛋白质印迹。在慢性初次感染中，仅在源自HLN的ASC探针中检测到寄生虫特异性抗体。二次感染7天后，在来自MLN的ASC探针中检测到有限的反应，主要针对新脱囊幼虫（NEJ）阶段特有的一种抗原。如果二次感染未被排斥且攻击的吸虫到达肝脏，这种NEJ特异性抗原才会被HLN识别。对ASC探针中存在的免疫球蛋白水平的测量显示，仅在近期感染引流部位的淋巴结中显著升高。此外，当确定源自不同淋巴结的ASC探针中的同种型谱时，观察到它们表现出不同的同种型偏好，特别是MLN偏好IgA，HLN偏好IgE，脾脏偏好IgM。这些结果表明，大鼠不同身体部位针对寄生虫不同阶段会发生离散且独立的免疫反应。
