Tsai F C, Gardner D K
Centre for Early Human Development, Monash University, Melbourne, Victoria, Australia.
Fertil Steril. 1994 Feb;61(2):376-82. doi: 10.1016/s0015-0282(16)56534-0.
To determine the effects of B-group vitamins present in culture media on mouse embryo development in vitro and subsequent viability.
Mouse zygotes were cultured in the presence of B-group vitamins. Embryo morphology and cell numbers were determined at 96 and 120 hours after hCG. Viability was assessed by transfer of embryos after 3 days of culture to pseudopregnant recipients. Resultant pregnancy rates (PRs) and fetal weights were determined.
Supplementation of an amino acid-free medium with minimal essential medium (MEM) B-group vitamins significantly decreased embryo cleavage rates, whereas the inclusion of Ham's F-10 medium B-group vitamins significantly reduced both cleavage rates and morphological development. Subsequent experiments determined that nicotinamide (5 microM) significantly reduced blastocyst cell number, implantation rate, viable PR, and fetal weight.
The data indicate that nicotinamide inhibits mouse embryo development in culture and reduces viability. Nicotinamide is present at high levels in Ham's F-10 and MEM media that are used routinely in human embryo culture. The role of vitamins in human embryo development in vitro warrants investigation.
确定培养基中B族维生素对小鼠胚胎体外发育及后续生存能力的影响。
将小鼠受精卵在B族维生素存在的情况下进行培养。在注射人绒毛膜促性腺激素（hCG）后96和120小时测定胚胎形态和细胞数量。通过将培养3天后的胚胎移植到假孕受体中来评估生存能力。测定由此产生的妊娠率（PRs）和胎儿体重。
在无氨基酸培养基中添加最低限度基本培养基（MEM）B族维生素显著降低胚胎分裂率，而添加哈姆氏F-10培养基B族维生素则显著降低分裂率和形态发育。后续实验确定烟酰胺（5微摩尔）显著减少囊胚细胞数量、着床率、活胎妊娠率和胎儿体重。
数据表明烟酰胺抑制培养中的小鼠胚胎发育并降低生存能力。烟酰胺在人类胚胎培养中常用的哈姆氏F-10和MEM培养基中含量很高。维生素在人类胚胎体外发育中的作用值得研究。
