Cone R E, Marchalonis J J
Department of Pathology, University of Connecticut Health Center, Farmington 06030-3105.
Immunol Invest. 1993 Dec;22(8):541-52. doi: 10.3109/08820139309084182.
Antigen-specific molecules secreted by murine T cell hybrids (TABM) specific for 4-hydroxy 3-nitrophenyl (NP) or azobenzenearsonate (ABA) were purified from ascitic fluid by ion exchange chromatography and/or affinity for antigen. Partial amino acid sequence of reduced Mr 72,000 NP-specific polypeptides and Mr 20,000 peptides prepared by treatment of the ABA-specific immunoprotein with cyanogen bromide was obtained and a septapeptide of the NP-specific TABM shared 3/7 residues with the ABA-specific TABM. Both TABM shared residues present in 95% T cell receptor for antigen (TCR) V alpha subgroup I and 83-96% murine immunoglobulin V kappa Fr3. These results provide evidence that extracellular antigen-specific T cell proteins are soluble analogues of TCR alpha chains and belong to the immunoglobulin supergene family.
从腹水通过离子交换色谱法和/或对抗原的亲和力纯化了对4-羟基-3-硝基苯基（NP）或偶氮苯砷酸盐（ABA）具有特异性的鼠T细胞杂交瘤分泌的抗原特异性分子（TABM）。获得了还原的分子量为72,000的NP特异性多肽以及通过用溴化氰处理ABA特异性免疫蛋白制备的分子量为20,000的肽的部分氨基酸序列，并且NP特异性TABM的一个七肽与ABA特异性TABM共享3/7个残基。两种TABM都共享存在于95%的抗原T细胞受体（TCR）Vα亚组I和83-96%的鼠免疫球蛋白Vκ Fr3中的残基。这些结果提供了证据，表明细胞外抗原特异性T细胞蛋白是TCRα链的可溶性类似物，并且属于免疫球蛋白超基因家族。
