一种在雌性斯普拉格-道利大鼠中连续传代和维持鼠类圆线虫同宿主型品系的替代方案。

An alternative protocol for the serial passage and maintenance of a homogonic strain of Strongyloides ratti in female Sprague-Dawley rats.

作者信息

Mendis A H, Grubb W B, Armson A

机构信息

School of Biomedical Sciences, Curtin University of Technology, Perth, Western Australia.

出版信息

Int J Parasitol. 1993 Sep;23(6):813-4. doi: 10.1016/0020-7519(93)90080-i.

DOI:10.1016/0020-7519(93)90080-i

PMID:8300292

Abstract

A method which does not involve the tedious use of watch glass coprocultures for obtaining filariform infective (L3) larvae of Strongyloides ratti from faecal pellets of infected Sprague-Dawley rats is described. The alternative method utilises Baermannization (18 h) of faecal pellets to yield rhabditiform (L1) larvae of S. ratti and their subsequent culture for 72 h at 19 degrees C in tissue-culture-flasks containing only dechlorinated tap water to yield infective filariform (L3) larvae. The yields and infectivity of the L3 larvae obtained from the two methods were essentially similar.

摘要

本文描述了一种方法，该方法不涉及使用培养皿进行繁琐的共培养，以从感染的斯普拉格-道利大鼠粪便颗粒中获取鼠类圆线虫丝状感染性（L3）幼虫。替代方法是对粪便颗粒进行贝氏分离法（18小时）以获得鼠类圆线虫的杆状（L1）幼虫，随后将其在仅含有去氯自来水的组织培养瓶中于19摄氏度培养72小时，以产生感染性丝状（L3）幼虫。从这两种方法获得的L3幼虫的产量和感染性基本相似。