Yoshimura K, Sugaya H, Ishida K, Khan W I, Abe T, Unno K
Department of Parasitology, Akita University School of Medicine, Japan.
Int J Parasitol. 1993 Dec;23(8):997-1003. doi: 10.1016/0020-7519(93)90119-j.
Male BALB/c mice were infected with Angiostrongylus cantonensis and at various times p.i. treated with cyclosporin A (CsA) either for 5 days continuously or intermittently, or for 12-16 days on alternate days. They were monitored for peripheral blood eosinophilia and at necropsy examined for CSF and bone marrow eosinophilia, and worm recovery. CsA treatment provoked a transient inhibition of peripheral blood eosinophilia in all groups examined, followed by rebounding eosinophilia. There was no significant difference in bone marrow and CSF eosinophilia between CsA- and vehicle-treated groups. Mice treated with CsA on alternate days yielded lower intracranial worm recovery with small-sized worms at days 7, 21 and 30 p.i. than vehicle-treated groups did. No significant reduction in worm recovery was noted in mice treated for 5 days either continuously or intermittently, although worms, especially female ones, harvested from groups treated with CsA from days--1 to 3 or from days 13 to 17 were significantly smaller than those from vehicle-treated groups. CsA-treatment suppressed blastogenic responses of spleen cells against Con A or worm antigen at days 7 and 21 p.i. In vitro treatment of the 3rd stage larvae with CsA did not adversely influence survival of A. cantonensis in mice. These data indicate that CsA exerts anti-parasitic effects against A. cantonensis in mice.
雄性BALB/c小鼠感染广州管圆线虫，在感染后的不同时间点，连续5天或间歇性地用环孢素A（CsA）治疗，或隔天治疗12 - 16天。监测外周血嗜酸性粒细胞增多情况，并在尸检时检查脑脊液和骨髓中的嗜酸性粒细胞增多情况以及虫体回收率。CsA治疗在所有检查的组中均引起外周血嗜酸性粒细胞增多的短暂抑制，随后嗜酸性粒细胞增多反弹。CsA治疗组和载体治疗组之间的骨髓和脑脊液嗜酸性粒细胞增多情况无显著差异。在感染后第7天、21天和30天，隔天用CsA治疗的小鼠颅内虫体回收率较低，且虫体较小，低于载体治疗组。连续或间歇性治疗5天的小鼠未观察到虫体回收率有显著降低，尽管从第 - 1天至第3天或第13天至第17天用CsA治疗的组中收获的虫体，尤其是雌虫，明显小于载体治疗组。在感染后第7天和21天，CsA治疗抑制了脾细胞对刀豆蛋白A或虫体抗原的增殖反应。用CsA对第三期幼虫进行体外处理对广州管圆线虫在小鼠体内的存活没有不利影响。这些数据表明CsA对小鼠体内的广州管圆线虫具有抗寄生虫作用。
