Sugaya H, Ishida K, Yoshimura K
Department of Parasitology, Akita University School of Medicine, Japan.
Int J Parasitol. 1997 Jan;27(1):113-7. doi: 10.1016/s0020-7519(96)00165-8.
Resistance to Angiostrongylus cantonensis is contingent upon the generation of an eosinophilic response in the CSF of infected hosts. We have studied the parameters required for the generation of this CSF eosinophilia in normally permissive rats. We initially induced a marked peripheral eosinophilia in rats by infection with either Mesocestoides corti or Angiostrongylus cantonensis or the surgical transfer of A. cantonensis young adult worms (YA) into their pulmonary arteries. Next, we injected various antigens into the ventricles of these rats. A. cantonensis-preinfected rats demonstrated significant CSF eosinophilia following injection of A. cantonensis egg antigen, 1st-stage larval (L1) antigen, or M. corti antigen, but not following YA antigen inoculation. A. cantonensis egg and M. corti antigens were potent chemoattractants for eosinophils in an in vitro chemotaxis assay. These data indicate that peripheral eosinophilia, meningeal stimulation by A. cantonensis infection and the presence of potent chemoattractants, e.g., egg and L1 antigens are prerequisites for CSF eosinophil accumulation in permissive rat hosts.
对广州管圆线虫的抵抗力取决于受感染宿主脑脊液中嗜酸性粒细胞反应的产生。我们研究了在通常易感染的大鼠中产生这种脑脊液嗜酸性粒细胞增多所需的参数。我们最初通过感染柯氏中绦虫或广州管圆线虫，或将广州管圆线虫成虫（YA）手术转移到大鼠肺动脉中，诱导大鼠出现明显的外周嗜酸性粒细胞增多。接下来，我们向这些大鼠的脑室注射各种抗原。预先感染广州管圆线虫的大鼠在注射广州管圆线虫卵抗原、第一期幼虫（L1）抗原或柯氏中绦虫抗原后，脑脊液嗜酸性粒细胞显著增多，但接种YA抗原后则没有。在体外趋化试验中，广州管圆线虫卵抗原和柯氏中绦虫抗原是嗜酸性粒细胞的有效趋化剂。这些数据表明，外周嗜酸性粒细胞增多、广州管圆线虫感染引起的脑膜刺激以及有效趋化剂（如卵抗原和L1抗原）的存在是易感染大鼠宿主脑脊液嗜酸性粒细胞积聚的先决条件。
