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通过质粒图谱和IS200分布对鼠伤寒沙门氏菌维尔乔噬菌体类型进行鉴定

Characterization of Salmonella virchow phage types by plasmid profile and IS200 distribution.

作者信息

Torre E, Threlfall E J, Hampton M D, Ward L R, Gibert I, Rowe B

机构信息

Department of Patología i Produccions Animals, Facultat de Veterinaria, Universitat Autònoma de Barcelona, Spain.

出版信息

J Appl Bacteriol. 1993 Nov;75(5):435-40. doi: 10.1111/j.1365-2672.1993.tb02799.x.

Abstract

The type strains of the 57 phage types of Salmonella virchow have been characterized by plasmid profile and by distribution of the insertion sequence IS200. Thirty-two strains carried plasmids and 21 profile types were identified; 17 strains were resistant to antimicrobial agents. In contrast only six of the type strains carried IS200 elements and three patterns were identified. Within Salm. virchow phage type 31, five of 10 wild-type isolates carried plasmids and two plasmid profiles were identified; in contrast, an IS200 element was identified in the genome of only one of these strains. It is concluded that for Salm. virchow, IS200 is unlikely to significantly extend the degree of discrimination achieved by phage typing which may be supplemented when appropriate by plasmid profile typing.

摘要

对57种维氏沙门氏菌噬菌体类型的模式菌株进行了质粒图谱分析和插入序列IS200分布特征分析。32株携带质粒,鉴定出21种图谱类型;17株对抗菌剂耐药。相比之下,只有6株模式菌株携带IS200元件,鉴定出3种模式。在维氏沙门氏菌噬菌体31型中,10株野生型分离株中有5株携带质粒,鉴定出2种质粒图谱类型;相比之下,这些菌株中只有1株的基因组中鉴定出IS200元件。结论是,对于维氏沙门氏菌,IS200不太可能显著扩展噬菌体分型所达到的鉴别程度,在适当的时候可通过质粒图谱分型进行补充。

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