Haginaka J, Murashima T, Fujima H, Wada H
Faculty of Pharmaceutical Sciences, Mukogawa Women's University, Nishinomiya, Japan.
J Chromatogr. 1993 Oct 29;620(2):199-204. doi: 10.1016/0378-4347(93)80004-n.
A high-performance liquid chromatographic (HPLC) method for the determination of drug enantiomers in serum was developed. The method involves direct injection of serum samples on to an ovomucoid-bonded column, which is prepared by bonding of ovomucoid proteins to an aminopropyl-silica gel by the N,N'-disuccinimidyl carbonate activation method and separation of drug enantiomers on the column using a mixture of phosphate buffer and an organic solvent. High recoveries of serum proteins were obtained using eluent pH values of 3, 4, 6 and 7 at phosphate buffer concentrations above 50 mM, whereas the recovery was ca. 70% at an eluent pH of 5. The recovery of each enantiomer of basic and acidic drugs from serum was almost 100%.
