Shin E K, Akamizu T, Matsuda F, Sugawa H, Fujikura J, Mori T, Honjo T
Department of Medical Chemistry, Faculty of Medicine, Kyoto University, Japan.
J Immunol. 1994 Feb 1;152(3):1485-92.
We have established EBV-transformed human B cell clones producing monoclonal antithyrotropin receptor antibodies from two patients with Graves' disease. We then isolated and characterized Ig H chain genes of 5 B cell clones with the thyrotropin-binding inhibitor Ig (TBII) activity and 4 B cell clones with the thyroid-stimulating antibody (TSAb) activity. We found that VH gene families used in the 5 TBII clones were all VH-III, although those of the four TSAb clones were diverse, including VH-II, -III, -IV, all -V. Most of VH segments used in TBII and TSAb are commonly used in other autoantibodies and fetal liver repertoire. The frequency of somatic mutations in TBII was higher than that in TSAb. Inasmuch as the same germline VH segment (V3-23) was used for both TBII and TSAb, the frequency and position of somatic mutations may be important for generation of TBII and TSAb.
我们已经建立了来自两名格雷夫斯病患者的EB病毒转化的人B细胞克隆，这些克隆产生抗促甲状腺素受体单克隆抗体。然后，我们分离并鉴定了5个具有促甲状腺素结合抑制性Ig（TBII）活性的B细胞克隆和4个具有促甲状腺素刺激抗体（TSAb）活性的B细胞克隆的Ig H链基因。我们发现，5个TBII克隆中使用的VH基因家族均为VH-III，而4个TSAb克隆的VH基因家族则各不相同，包括VH-II、-III、-IV和-V。TBII和TSAb中使用的大多数VH区段在其他自身抗体和胎儿肝脏库中也常用。TBII中体细胞突变的频率高于TSAb。由于TBII和TSAb使用相同的种系VH区段（V3-23），体细胞突变的频率和位置可能对TBII和TSAb的产生很重要。
