Stroncek D F, Herr G P, Plachta L B
American Red Cross St. Paul Regional Blood Services, MN.
J Lab Clin Med. 1994 Feb;123(2):247-55.
Neutrophil-specific antigen NB1 is located on a 58 to 64 kd glycosyl phosphatidylinositol-linked plasma membrane glycoprotein. NB1 antigen can be detected on neutrophils from 97% of healthy volunteers, and NB1 antigen is expressed on subpopulations of neutrophils. Neutrophil subpopulations with varying functions have been described, and we hypothesize that NB1 antigen may play an important role in neutrophil function. We compared the function of NB1-positive and NB1-negative neutrophils obtained from several persons. There were no differences in the adhesion of NB1-positive and NB1-negative neutrophils incubated in C5a, N-formyl-Met-Leu-Phe (FMLP), phorbol myristate acetate (PMA), or buffer to type IV collagen, fibronectin, laminin, or polystyrene. However, the adherence to human umbilical vein endothelial cells (HUVEC) monolayers of unstimulated NB1-positive neutrophils was less than to NB1-negative neutrophils (20.0% +/- 4.2% vs 31.7% +/- 5.8%; p < 0.01). When neutrophils were stimulated with C5a, PMA, or FMLP, no differences were found in the adhesion of NB1-positive and NB1-negative cells to the same surfaces. When NB1-positive neutrophils were incubated with rabbit polyclonal anti-NB1 Fab fragments, their adherence to HUVEC was increased (32.9% +/- 10.1% vs 18.3% +/- 5.0%; p < 0.05). Fab fragments prepared from normal rabbit serum had no effect on neutrophil adherence to HUVEC. The chemotaxis of NB1-positive neutrophils to FMLP through nitrocellulose was significantly greater than that of NB1-negative neutrophils (p = 0.03), but there was no difference in chemotaxis to FMLP through polycarbonate membranes.(ABSTRACT TRUNCATED AT 250 WORDS)
中性粒细胞特异性抗原NB1位于一种58至64kd的糖基磷脂酰肌醇连接的质膜糖蛋白上。在97%的健康志愿者的中性粒细胞上可检测到NB1抗原，且NB1抗原在中性粒细胞亚群上表达。已经描述了具有不同功能的中性粒细胞亚群，我们推测NB1抗原可能在中性粒细胞功能中起重要作用。我们比较了从几个人获得的NB1阳性和NB1阴性中性粒细胞的功能。在C5a、N-甲酰甲硫氨酰亮氨酰苯丙氨酸（FMLP）、佛波酯肉豆蔻酸酯乙酸酯（PMA）或缓冲液中孵育的NB1阳性和NB1阴性中性粒细胞与IV型胶原、纤连蛋白、层粘连蛋白或聚苯乙烯的黏附没有差异。然而，未刺激的NB1阳性中性粒细胞对人脐静脉内皮细胞（HUVEC）单层的黏附低于NB1阴性中性粒细胞（20.0%±4.2%对31.7%±5.8%；p<0.01）。当中性粒细胞用C5a、PMA或FMLP刺激时，NB1阳性和NB1阴性细胞对相同表面的黏附没有差异。当NB1阳性中性粒细胞与兔多克隆抗NB1 Fab片段孵育时，它们对HUVEC的黏附增加（32.9%±10.1%对18.3%±5.0%；p<0.05）。从正常兔血清制备的Fab片段对中性粒细胞黏附HUVEC没有影响。NB1阳性中性粒细胞通过硝酸纤维素对FMLP的趋化作用明显大于NB1阴性中性粒细胞（p = 0.03），但通过聚碳酸酯膜对FMLP的趋化作用没有差异。（摘要截断于250字）
