Miller A D, Ruggiero D A
Rockefeller University, New York, New York 10021.
J Neurosci. 1994 Feb;14(2):871-88. doi: 10.1523/JNEUROSCI.14-02-00871.1994.
The organization of the central neuronal circuitry that produces vomiting was explored by mapping the distribution of c-fos protein (Fos)-like immunoreactivity (FLI) as a monitor of functional activity. The brainstem and spinal cord were examined in cats administered multiple emetic drugs (cisplatin, lobeline, protoveratrine, naloxone, apomorphine) or control saline injections. Some animals were decerebrated, paralyzed, and artificially ventilated to avoid possible Fos expression induced by sensory feedback or fluid depletion during vomiting. Fictive vomiting was identified in these animals by a characteristic pattern of respiratory muscle nerve (phrenic and abdominal) coactivation. Tissues were immunoprocessed using an antibody raised against amino acids 1-131 of Fos and the avidin-biotin peroxidase complex method. Enhanced nuclear FLI was observed in experimental animals along portions of the sensorimotor emetic reflex arc, including the nodose ganglia, area postrema, nuclei of the solitary tract (especially medial and subpostrema subnuclei), intermediate reticular zone of the lateral tegmental field, nucleus retroambiguus, C2 inspiratory propriospinal cell region, and dorsal vagal and phrenic motor nuclei. Enhanced FLI was also detected in the raphe magnus, subretrofacial nucleus, and spinal dorsal horn. Regions showing no recognizable differences in FLI between experimental and control animals included the vestibular, cochlear, spinal trigeminal, subtrigeminal, and lateral reticular nuclei. Only minor differences were observed in the distributions of FLI between intact and decerebrate animals. No unique, well-defined group of labeled neurons that might function as a "vomiting center" could be identified. Instead, the pattern of c-fos expression suggests that neurons involved in coordinating the emetic response may radiate from the area postrema and nucleus of the solitary tract to an arc in the lateral tegmental field implicated in somato-autonomic integration.
通过绘制c-fos蛋白（Fos）样免疫反应性（FLI）的分布来监测功能活动，从而探究产生呕吐的中枢神经回路的组织情况。对接受多种催吐药物（顺铂、洛贝林、原藜芦碱、纳洛酮、阿扑吗啡）或对照生理盐水注射的猫的脑干和脊髓进行了检查。一些动物进行了去大脑、麻痹和人工通气处理，以避免呕吐期间感觉反馈或液体消耗诱导的可能的Fos表达。通过呼吸肌神经（膈神经和腹神经）共同激活的特征模式在这些动物中识别出假性呕吐。使用针对Fos的1-131氨基酸产生的抗体和抗生物素蛋白-生物素过氧化物酶复合物方法对组织进行免疫处理。在实验动物中，沿着感觉运动催吐反射弧的部分区域观察到增强的核FLI，包括结节神经节、最后区、孤束核（特别是内侧和最后区亚核）、外侧被盖区的中间网状带、疑后核、C2吸气脊髓 propriospinal细胞区域以及迷走背核和膈运动核。在中缝大核、面后核和脊髓背角也检测到增强的FLI。在实验动物和对照动物之间FLI没有可识别差异的区域包括前庭核、耳蜗核、脊髓三叉神经核、三叉下核和外侧网状核。在完整动物和去大脑动物之间，FLI的分布仅观察到微小差异。未发现可能作为“呕吐中枢”发挥作用的独特、明确的标记神经元群。相反，c-fos表达模式表明，参与协调催吐反应的神经元可能从最后区和孤束核辐射到外侧被盖区中与躯体-自主神经整合有关的一个弧。
