Landweber L F, Gilbert W
Department of Cellular and Developmental Biology, Harvard University Biological Laboratories, Cambridge, MA 02138.
Proc Natl Acad Sci U S A. 1994 Feb 1;91(3):918-21. doi: 10.1073/pnas.91.3.918.
RNA editing by extensive uridine addition and deletion creates over 90% of the amino acid codons in the cytochrome-c oxidase subunit III (COIII) transcript in Trypanosoma brucei and Herpetomonas, whereas editing of the COIII transcripts in Leishmania tarentolae and Crithidia fasciculata generates only 6% of the amino acid codons and is limited to the 5' ends. Is extensive RNA editing a primitive or derived character? We constructed a phylogenetic tree based on nuclear small-subunit and mitochondrial large- and small-subunit ribosomal RNA sequences for nine species of kinetoplastid protozoa. Our results suggest that extensive editing is a primitive genetic phenomenon that has disappeared in recent evolutionary time and also that there have been multiple losses of the digenetic lifestyle by loss of the vertebrate host in parasite evolution.
通过大量添加和删除尿苷进行的RNA编辑，在布氏锥虫和赫氏鞭毛虫的细胞色素c氧化酶亚基III（COIII）转录本中产生了超过90%的氨基酸密码子，而在大利什曼原虫和 fasciculata 短膜虫中，COIII转录本的编辑仅产生6%的氨基酸密码子，并且仅限于5'端。广泛的RNA编辑是一种原始特征还是衍生特征？我们基于九种动基体原生动物的核小亚基以及线粒体大、小亚基核糖体RNA序列构建了系统发育树。我们的结果表明，广泛编辑是一种原始的遗传现象，在最近的进化过程中已经消失，并且在寄生虫进化过程中，由于脊椎动物宿主的丧失，双宿主生活方式也多次丧失。
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