Schimmang T, van der Hoeven F, Rüther U
European Molecular Biology Laboratory, Heidelberg, Germany.
Prog Clin Biol Res. 1993;383A:153-61.
We investigated the relation between the recessive mutation add caused by insertional mutagenesis of a transgene and the dominant mutation extra-toes (Xt) which display similar effects on limb morphogenesis. The transgene insertion in add mapped close to the 5' end of the zinc finger gene Gli3 and 5'sequences of Gli3 were deleted in Xt. Expression analysis revealed a reduction of the Gli3 RNA level in both add and Xt mice. Thus, it is very likely that add and Xt are alleles of the Gli3 gene.
我们研究了转基因插入诱变引起的隐性突变add与显性突变多趾（Xt）之间的关系，这两种突变在肢体形态发生上表现出相似的效应。add中转基因插入位点靠近锌指基因Gli3的5'端，且Xt中Gli3的5'序列缺失。表达分析显示add和Xt小鼠中Gli3 RNA水平均降低。因此，add和Xt很可能是Gli3基因的等位基因。
