Abbott U K, Pisenti J M
Department of Avian Sciences, University of California, Davis 95616.
Prog Clin Biol Res. 1993;383A:99-112.
Classical studies of the vertebrate limb have provided a firm foundation for recent investigations into the molecular control of mechanisms governing limb patterning. The early studies revealed the importance of inductive tissue interactions in developing systems, the spatiotemporal restrictions of these interactions, and the conservation of inductive signals between different tissues and even different species. They incorporated a number of different experimental approaches, including: homologous and heterologous tissue grafting and recombination, the investigation of several limb mutations, and examination of the response of normal limb tissue to a variety of teratogenic treatments. While some of the mutations studied only affected the limbs, most were highly pleiotropic, producing complex syndromes that altered the development of several embryonic structures in addition to the limbs. Some of these syndromes could be partially or completely phenocopied (mimicked) by specific chemical or physical treatments. One such gene-phenocopy pairing that we have studied is that of the mutation wingless-2 and the syndrome produced by treatment with retinoic acid. Another aspect of abnormal pattern formation we explored is the interaction between wingless-2 and eudiplopodia.
对脊椎动物肢体的经典研究为近期关于肢体模式形成机制的分子控制研究奠定了坚实基础。早期研究揭示了诱导性组织相互作用在发育系统中的重要性、这些相互作用的时空限制，以及不同组织甚至不同物种之间诱导信号的保守性。这些研究采用了多种不同的实验方法，包括：同源和异源组织移植与重组、对多种肢体突变的研究，以及对正常肢体组织对各种致畸处理反应的检查。虽然所研究的一些突变仅影响肢体，但大多数具有高度多效性，会产生复杂的综合征，除了肢体之外还会改变几个胚胎结构的发育。其中一些综合征可以通过特定的化学或物理处理部分或完全模拟（模仿）。我们研究过的一个这样的基因-表型模拟配对是无翅-2突变与视黄酸处理产生的综合征。我们探索的异常模式形成的另一个方面是无翅-2与双足畸形之间的相互作用。
