马红球菌毒力相关质粒的限制酶切图谱。
Restriction map of a virulence-associated plasmid of Rhodococcus equi.
作者信息
Kanno T, Asawa T, Ito H, Takai S, Tsubaki S, Sekizaki T
机构信息
National Institute of Animal Health, Ibaraki, Japan.
出版信息
Plasmid. 1993 Nov;30(3):309-11. doi: 10.1006/plas.1993.1065.
PMID:8302941
Abstract
The restriction cleavage map of a virulence-associated plasmid pREAT701 of Rhodococcus equi was constructed with EcoRI and HindIII by means of cloning the restriction fragments, cross Southern hybridization with each fragment, and hybridization with probes generated by modified inverse PCR. The genetic region responsible for expression of virulence-associated 15- to 17-kilodalton antigens was determined.
摘要
通过克隆限制性片段、各片段间的交叉Southern杂交以及与改良反向PCR产生的探针杂交，用EcoRI和HindIII构建了马红球菌毒力相关质粒pREAT701的限制性酶切图谱。确定了负责毒力相关15至17千道尔顿抗原表达的基因区域。
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