橙皮素对大鼠脂肪细胞的抗脂解作用。
Antilipolytic action of hesperetin in rat adipocytes.
作者信息
Kuppusamy U R, Das N P
机构信息
Department of Biochemistry, National University of Singapore.
出版信息
Planta Med. 1993 Dec;59(6):508-12. doi: 10.1055/s-2006-959749.
Hesperetin was able to inhibit both epinephrine- and theophylline-induced lipolysis in rat adipocytes. At concentrations of 125 microM and 250 microM, it could augment the inhibitory effect of low insulin concentration (17 microU/2ml) on epinephrine-induced lipolysis. The basal cyclic AMP levels and phosphodiesterase activities in intact adipocytes were unaltered by hesperetin. The antilipolytic action of hesperetin in adenosine deaminase pretreated adipocytes was abolished. Hesperetin could only prevent lipolysis but not reverse the initial lipolytic activity stimulated through the adenosine deaminase pretreatment of the adipocytes. In pertussis or cholera toxin (PT or CT) pretreated adipocytes, the antilipolytic action of hesperetin was unaffected. From the data obtained it is possible to conclude that the antilipolytic action of hesperetin is not mediated through the inhibition of cyclic AMP level nor through CT- or PT-sensitive G proteins. However, the involvement of hesperetin in preventing the accessibility of cellular receptors to the actions of lipolytic agents may be implicated.
橙皮素能够抑制大鼠脂肪细胞中肾上腺素和茶碱诱导的脂肪分解。在125微摩尔/升和250微摩尔/升的浓度下，它可以增强低胰岛素浓度（17微单位/2毫升）对肾上腺素诱导的脂肪分解的抑制作用。橙皮素对完整脂肪细胞中的基础环磷酸腺苷水平和磷酸二酯酶活性没有影响。橙皮素在经腺苷脱氨酶预处理的脂肪细胞中的抗脂解作用被消除。橙皮素只能阻止脂肪分解，但不能逆转通过对脂肪细胞进行腺苷脱氨酶预处理所刺激的初始脂肪分解活性。在百日咳毒素或霍乱毒素（PT或CT）预处理的脂肪细胞中，橙皮素的抗脂解作用不受影响。从获得的数据可以得出结论，橙皮素的抗脂解作用不是通过抑制环磷酸腺苷水平，也不是通过对CT或PT敏感的G蛋白介导的。然而，可能涉及橙皮素在阻止细胞受体接触脂解剂作用方面的作用。
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