Giorgi R, Bernardi M M, Cury Y
Laboratory of Pathophysiology, Butantan Institute, São Paulo, Brazil.
Toxicon. 1993 Oct;31(10):1257-65. doi: 10.1016/0041-0101(93)90399-4.
Crude venom obtained from Crotalus durissus terrificus (Cdt) was tested for its possible analgesic effect in mice. Subcutaneous (s.c.), intraperitoneal (i.p.) or oral (p.o.) administration of the venom caused an antinociceptive effect in mice as measured by the acetic acid-induced writhing method and the hot plate test. The antinociceptive activity was dose and time dependent and persisted after neutralization of the venom with a specific antivenin. It was demonstrated that the factor(s) has an apparent mol. wt of less than 3000 and that its antinociceptive effect is abolished by trypsin treatment. The demonstration that morphine enhances the analgesic effect of Cdt venom and naloxone antagonizes this effect suggests an endorphin-like activity for the factor(s) herein described.
对从剧毒矛头蝮（Cdt）获取的粗毒液进行了小鼠镇痛效果测试。通过乙酸诱导扭体法和热板试验测定，皮下（s.c.）、腹腔内（i.p.）或口服（p.o.）给予毒液均可使小鼠产生抗伤害感受作用。抗伤害感受活性呈剂量和时间依赖性，并且在用特异性抗蛇毒血清中和毒液后仍持续存在。结果表明，该因子的表观分子量小于3000，且其抗伤害感受作用可被胰蛋白酶处理消除。吗啡增强Cdt毒液的镇痛作用以及纳洛酮拮抗该作用的结果表明，本文所述因子具有类内啡肽活性。
