一种能够保护松鼠猴抵御无性血液期疟原虫的恶性疟原虫蛋白质组分（90 - 110 kDa）的免疫原性和抗原性。

Immunogenicity and antigenicity of a Plasmodium falciparum protein fraction (90-110 kDa) able to protect squirrel monkeys against asexual blood stages.

作者信息

Bonnefoy S, Gysin J, Blisnick T, Guillotte M, Carcy B, Pereira da Silva L, Mercereau-Puijalon O

机构信息

Unité de Parasitologie Expérimentale, Institut Pasteur, Paris, France.

出版信息

Vaccine. 1994 Jan;12(1):32-40. doi: 10.1016/0264-410x(94)90008-6.

DOI:10.1016/0264-410x(94)90008-6

PMID:8303938

Abstract

A monkey vaccination trial using a Plasmodium falciparum protein fraction containing antigens of 90-110 kDa is reported. The fraction was obtained by electroelution from preparative polyacrylamide gels. Three monkeys out of five resisted a heavy challenge dose of highly virulent parasites. Using specific antisera, several components of the fraction were identified, namely heat shock protein 90 (hsp90), Ag44/RhopH3, ABRA, 96tR/GBP130 and Pf96 protease. The fraction did not contain KAHRP, nor the SERP antigen. The antibody response of the monkeys was studied on these individual antigens purified by preparative immunoprecipitation. Surprisingly, hsp90 was found in the immunoprecipitates obtained with SERP antisera. Interestingly, the response to hsp90 correlated with protection, high antibody titres being found only in the protected monkeys. In contrast, no correlation with protection could be found for the response to the other antigens.

摘要

报道了一项使用含有90 - 110 kDa抗原的恶性疟原虫蛋白组分进行的猴子疫苗接种试验。该组分通过从制备型聚丙烯酰胺凝胶中电洗脱获得。五只猴子中有三只抵御了高毒力寄生虫的大量攻击剂量。使用特异性抗血清，鉴定出该组分的几个成分，即热休克蛋白90（hsp90）、Ag44/RhopH3、ABRA、96tR/GBP130和Pf96蛋白酶。该组分不含KAHRP，也不含SERP抗原。通过制备型免疫沉淀纯化这些单个抗原后，研究了猴子的抗体反应。令人惊讶的是，在用SERP抗血清获得的免疫沉淀物中发现了hsp90。有趣的是，对hsp90的反应与保护作用相关，仅在受保护的猴子中发现高抗体滴度。相比之下，对其他抗原的反应与保护作用没有相关性。