Kátó L, Szejtli J, Szente L
Catherine Booth Hospital, Montreal, Canada.
Acta Microbiol Hung. 1993;40(1):47-58.
Palmitic acid and palmitates were transformed into water soluble complexes with crystalline heptakis-2,6-di-0-methyl-beta-cyclodextrin. This formulation was incorporated into liquid and solid chemically well-defined media. The fatty acid served as C and energy source, ammonium thioglycolate as the sole source of N with the SH group as further source of energy. Minute amount of dimethyl-sulfoxide added was used for its known effect on cell membrane permeability. The media were inoculated with host grown Mycobacterium leprae cells isolated from human, armadillo and Nu mice foot pad lepromata. No growth occurred in the liquid medium at 22 or 32 degrees C, but cultures and subcultures of acid fast rods were grown at 10 degrees C. Bacilli in the cultures were solid, strongly acid fast rods, growing in clumps like globi. Growth on the semisolid media was visible as smooth round colonies, of white to ivory in colour, slowly expanding flatly at the periphery of the colony on the agar surface. Colonies developed within 2-3 weeks and reached maximum size at 50-80 days depending on the size of inoculum. Subcultures grow faster and more abundantly with adaptation to the media. No growth was seen without the water soluble complexes of palmitic acid or palmitates in the media. The free fatty acid or its salts had an equal growth supporting effect. Identical psychrophilic cultures were obtained from 7 out of 9 armadillo, 12 out of 12 Nu mice and 1 out of 2 human lepromata. None of the cultures grow on Loewenstein, Dubos or 7H9 media at 10 degrees C, 20 degrees C or 32 degrees C, respectively. The tested 4th to 7th subcultures of the strains were strongly positive for phenolic glycolipid-1. Heat killed suspensions of up to 7th subcultures gave negative late skin reaction in all of 16 LL cases. In 19 I, B and T cases the late skin reactions were all similar to that obtained with authentic human lepromin.
棕榈酸和棕榈酸盐与结晶七 - 2,6 - 二 - O - 甲基 - β - 环糊精形成水溶性复合物。该制剂被加入到液体和固体化学组成明确的培养基中。脂肪酸作为碳源和能源，硫代乙醇酸铵作为唯一的氮源，其巯基作为额外的能源。添加微量的二甲基亚砜是利用其对细胞膜通透性的已知作用。培养基接种从人、犰狳和裸鼠足垫麻风瘤中分离培养的麻风分枝杆菌细胞。在22℃或32℃的液体培养基中无生长，但在10℃时培养物和传代培养物中有抗酸杆菌生长。培养物中的杆菌为坚实、强抗酸杆菌，成丛生长如球团状。在半固体培养基上生长可见为光滑圆形菌落，颜色从白色到象牙色，在琼脂表面菌落周边缓慢平坦扩展。菌落2 - 3周内形成，根据接种量大小，50 - 80天达到最大尺寸。传代培养随着对培养基的适应生长更快且更旺盛。培养基中没有棕榈酸或棕榈酸盐的水溶性复合物则无生长。游离脂肪酸或其盐具有同等的生长支持作用。从9只犰狳中的7只、12只裸鼠中的12只以及2个人类麻风瘤中的1只获得了相同的嗜冷培养物。这些培养物在罗 - 琴培养基、杜波斯培养基或7H9培养基上，分别在10℃、20℃或32℃均不生长。测试的菌株第4至第7代传代培养物对酚糖脂 - 1呈强阳性。高达第7代传代培养物的热灭活悬液在16例LL病例中均产生阴性迟发性皮肤反应。在19例I、B和T病例中，迟发性皮肤反应均与用人麻风菌素获得的反应相似。
