Shivers R R, Chauvin W J
Cell Tissue Res. 1977 Jan 4;175(4):425-38. doi: 10.1007/BF00222410.
Labyrinth and nephridial canal cells of the crayfish (Orconectes virilis) antennal gland possess two types of intercellular junctions revealed by freeze-fracture studies. Apical margins of the cells are connected by long septate junctions. In replicas, these junctions consist of many parallel rows of 80--140 A intramembrane particles situated on the PF membrane face (EF and PF fracture faces of Branton et al., 1975). Rows of pits are found on the EF fracture face and are deemed complementary to the rows of particles. Moreover, lateral margins of basal regions of the epithelial cells are attached by many intercellular junctions. These contacts are characterized in thin plastic sections by a narrow dense cytoplasmic plaque located subjacent to the plasma membrane at sites of adjoined cells, and 5 to 12 fine strands of dense material that extend across the intercellular gap between adjoined cells. In freeze-fracture replicas, EF intramembrane faces basal to the region of the plasma membrane containing septate junctions exhibit numerous discoid clusters of particles. The particle aggregates, assumed to represent freeze-cleave images of adhering junctions, range from 900 to 3,700 A in diameter, with individual particles about 185 A in diameter. These junctions appear to connect epithelial cell processes formed by basal infoldings of the plasmalemma, and occur between adiacent cells as well as adiacent processes of a single cell. The discrete aggregates of particles resemble replicated desmosomes (Shienvold and Kelly, 1974) and hemi-desmosomes (Shivers, 1976); therefore, they probably do not constitute a basis for electrical coupling between antennal gland epithelial cells.
小龙虾（奥氏原螯虾）触角腺的迷路和肾管细胞具有通过冷冻断裂研究揭示的两种细胞间连接类型。细胞的顶端边缘通过长的分隔连接相连。在复制品中，这些连接由位于PF膜面（Branton等人，1975年的EF和PF断裂面）上的许多平行排的80 - 140埃膜内颗粒组成。在EF断裂面上发现了成排的凹坑，被认为与颗粒排互补。此外，上皮细胞基部区域的外侧边缘通过许多细胞间连接相连。在薄塑料切片中，这些接触的特征是在相邻细胞的部位，紧贴质膜下方有一个狭窄的致密细胞质斑块，以及5到12条细的致密物质链，它们延伸穿过相邻细胞之间的细胞间隙。在冷冻断裂复制品中，质膜中含有分隔连接区域基部的EF膜内面显示出许多盘状颗粒簇。这些颗粒聚集体被认为代表粘着连接的冷冻劈裂图像，直径从900到3700埃不等，单个颗粒直径约为185埃。这些连接似乎连接了由质膜基部褶皱形成的上皮细胞突起，并且出现在相邻细胞之间以及单个细胞的相邻突起之间。这些离散的颗粒聚集体类似于复制的桥粒（Shienvold和Kelly，1974年）和半桥粒（Shivers，1976年）；因此，它们可能不构成触角腺上皮细胞之间电耦合的基础。
