使用聚合酶链反应作为落基山斑疹热的诊断测试。

The use of a polymerase chain reaction as a diagnostic test for Rocky Mountain spotted fever.

作者信息

Sexton D J, Kanj S S, Wilson K, Corey G R, Hegarty B C, Levy M G, Breitschwerdt E B

机构信息

Department of Medicine, Duke University Medical Center, Durham, North Carolina.

出版信息

Am J Trop Med Hyg. 1994 Jan;50(1):59-63.

PMID:8304573

Abstract

A polymerase chain reaction (PCR) for amplifying ribosomal DNA of Rickettsia rickettsii was performed on blood clots and urine samples from 10 patients with suspected Rocky Mountain spotted fever (RMSF) and five controls with nonrickettsial diseases. The results of this PCR-based procedure were positive in four of the five patients with probable RMSF, but reamplification was required in three patients. Rickettsia rickettsii was grown from the blood of two of these four patients. The urine from one patient was also PCR-positive. These results confirm earlier findings that the PCR can detect R. rickettsii, but the need for reamplification indicates that the lack of sensitivity is a serious limitation in the usefulness of the PCR as a clinical diagnostic test.

摘要

对10例疑似落基山斑疹热（RMSF）患者的血凝块和尿液样本以及5例非立克次体病对照样本进行了用于扩增立氏立克次体核糖体DNA的聚合酶链反应（PCR）。基于该PCR方法的检测结果显示，5例可能患有RMSF的患者中有4例呈阳性，但其中3例患者需要再次扩增。这4例患者中有2例血液培养出立氏立克次体。1例患者的尿液PCR检测也呈阳性。这些结果证实了早期的发现，即PCR能够检测到立氏立克次体，但需要再次扩增表明灵敏度不足是PCR作为临床诊断检测方法实用性的一个严重局限。