Avidin-biotin based electrochemical immunoassay for thyrotropin.
作者信息
Athey D, Ball M, McNeil C J
机构信息
Department of Clinical Biochemistry, Medical School, University of Newcastle Upon Tyne, UK.
出版信息
Ann Clin Biochem. 1993 Nov;30 ( Pt 6):570-7. doi: 10.1177/000456329303000608.
PMID:8304726
Abstract
A two site electrochemical enzyme immunoassay for thyrotropin (TSH) has been developed. This assay is based on the use of an immobilized capture antibody and a biotinylated second antibody. Detection is achieved via avidin labelled with alkaline phosphatase. The substrate 1-naphthyl phosphate was used and the product 1-naphthol was detected at disposable screen-printed carbon 8-electrode combs using specially designed instrumentation.
摘要
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