Pomerol Monseny J M, García José F, Nolasco Barri P, Veiga Lluch A, Calderón de Oya G, Parera Junyent N, Coroleu Lletget B V, Aran Corbella B
Departamento de Obstetricia y Ginecología, Instituto Universitario Dexeus, Barcelona, España.
Arch Esp Urol. 1993 Nov;46(9):811-5.
The possibility of aspirating sperm from the first segments of the seminal tract by microsurgical techniques has permitted in vitro fertilization of the oocytes of the partners of men with obstructive azoospermia and ejaculation disorders. Sperm can be aspirated from the epididymis by microsurgical techniques in those cases with deferent duct agenesis or other obstructive pathologies, or from the deferent duct if spermatogenesis is unimpaired and there is no epididymal obstruction, but spermatozoa do not reach the ejaculate. We previously reported a pregnancy achieved by in vitro fertilization using sperm aspirated from the epididymis and the present article reports the first pregnancy achieved in our country using sperm aspirated from the deferent duct of the spouse with aspermia of psychogenic origin. A micropipette was utilized to obtain the sample, which was prepared using the mini-Percoll method. A total of 10 million motile spermatozoa were obtained and 6 of the 15 oocytes aspirated from the spouse were fertilized. The 3 embryos transferred to the fallopian tube resulted in a twin pregnancy that was carried to term.
通过显微外科技术从精道前段吸取精子的可能性，使得对患有梗阻性无精子症和射精障碍男性的配偶的卵母细胞进行体外受精成为可能。在那些存在输精管发育不全或其他梗阻性病变的情况下，可通过显微外科技术从附睾吸取精子；如果生精功能未受损且附睾无梗阻，但精子无法进入射精液，则可从输精管吸取精子。我们之前报道了使用从附睾吸取的精子进行体外受精而实现的妊娠，本文报道了我国首例使用从患有心因性无精子症配偶的输精管吸取的精子而实现的妊娠。使用微量移液器获取样本，并采用微型Percoll方法进行样本制备。共获得1000万个活动精子，从配偶体内吸取的15个卵母细胞中有6个受精。移植到输卵管的3个胚胎导致了双胎妊娠并足月分娩。
