钾离子载体抑制大鼠肾上腺嗜铬细胞瘤PC12细胞中神经生长因子诱导的神经元分化。

K+ ionophores inhibit nerve growth factor-induced neuronal differentiation in rat adrenal pheochromocytoma PC12 cells.

作者信息

Harada H, Morita M, Suketa Y

机构信息

Department of Environmental Biochemistry and Toxicology, University of Shizuoka, School of Pharmaceutical Science, Japan.

出版信息

Biochim Biophys Acta. 1994 Feb 17;1220(3):310-4. doi: 10.1016/0167-4889(94)90154-6.

DOI:10.1016/0167-4889(94)90154-6

PMID:8305504

Abstract

Incubation with a K+/H+ ionophore nigericin attenuated the nerve growth factor (NGF)-induced neurite outgrowth in rat pheochromocytoma PC12 cells. However, a Na+/H+ ionophore monensin and a Ca2+ ionophore A23187 did not inhibited the neurite outgrowth. Nigericin also inhibited the NGF-caused induction of acetylcholinesterase and suppression of cell proliferation. These changes were dependent on the amount of the ionophore added to the culture. In addition, a distinct K+ ionophore, valinomycin, similarly inhibited the NGF-induced neuronal differentiation. These results suggest the presence of the K+ ionophore-sensitive mechanism in the NGF-induced differentiation system in PC12 cells.

摘要

用钾离子/氢离子载体尼日利亚菌素孵育可减弱神经生长因子（NGF）诱导的大鼠嗜铬细胞瘤PC12细胞的神经突生长。然而，钠离子/氢离子载体莫能菌素和钙离子载体A23187并未抑制神经突生长。尼日利亚菌素还抑制了NGF引起的乙酰胆碱酯酶诱导和细胞增殖抑制。这些变化取决于添加到培养物中的离子载体的量。此外，一种独特的钾离子载体缬氨霉素同样抑制了NGF诱导的神经元分化。这些结果表明在PC12细胞的NGF诱导分化系统中存在钾离子载体敏感机制。