Hu Z Z, Buczko E, Zhuang L, Dufau M L
Section on Molecular Endocrinology, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892.
Biochim Biophys Acta. 1994 Feb 17;1220(3):333-7. doi: 10.1016/0167-4889(94)90158-9.
We present 6.2 kb of the 3'-noncoding region sequence of the rat luteinizing hormone receptor (LHR) gene and identification of two functional polyadenylation (pA) domains, H1 (nt 2368-2491) and H2 (nt 5579-5768) responsible for 3'-end processing of the 2.6/2.3 kb and the 5.8 kb LHR mRNA, respectively. Two identical copies of pA elements AAUAUA in H1 and of AAUAAA in H2 account for micro-heterogeneous poly(A) addition at each of the two pA regions. Both LH holoreceptor and major splice variant form B (lacking the first 266 bp of exon 11) are identified in H1-terminated (2.6 kb and 2.3 kb) and H2-terminated (5.8 kb) mRNA transcripts. A rodent repetitive DNA LINE R domain 3' of H1 within the major 5.8 kb species and a B2 element downstream of H2 were identified. Alignment of the 3'-noncoding region of LHR with TSH, FSH and beta 2-adrenergic receptors indicate that H1 pA signal is unique to the LHR and may represent an insertion domain.
我们展示了大鼠促黄体生成素受体（LHR）基因3'-非编码区6.2 kb的序列，并鉴定出两个功能性聚腺苷酸化（pA）结构域，即H1（核苷酸2368 - 2491）和H2（核苷酸5579 - 5768），它们分别负责2.6/2.3 kb和5.8 kb LHR mRNA的3'-末端加工。H1中的pA元件AAUAUA和H2中的AAUAAA的两个相同拷贝分别导致在两个pA区域的每个区域出现微异质性聚腺苷酸化。在H1末端（2.6 kb和2.3 kb）和H2末端（5.8 kb）的mRNA转录本中均鉴定出促黄体生成素全受体和主要剪接变体形式B（缺少外显子11的前266 bp）。在主要的5.8 kb物种中，H1内的一个啮齿动物重复DNA LINE R结构域位于3'端，并且在H2下游鉴定出一个B2元件。LHR的3'-非编码区与促甲状腺激素、促卵泡激素和β2 - 肾上腺素能受体的比对表明，H1 pA信号是LHR所特有的，可能代表一个插入结构域。
