Planes A M, Gasser I, González T, Ramírez A, Arcalís L
Servicio de Microbiología, Ciutat Universitària de la Vall d'Hebron, Barcelona.
Enferm Infecc Microbiol Clin. 1993 Nov;11(9):494-6.
To assess the utility of Bactec non-radiometric system in detecting disseminated mycobacteremia.
Blood cultures are processed using the Bactec NR 660/HPS system. Incubation of the NR6A vials, from HIV infected patients, is prolonged for 6 weeks and read once a week. After the 14th day of incubation a growth value > or = 30 is considered positive.
Mycobacteria were recovered from 80 blood cultures from 32 human immunodeficiency virus infected patients. The mean time required to detect growth was 37.8 days (range 20-45). Mycobacterium tuberculosis was isolated from 18 patients and Mycobacterium avium from 14. In 12 patients (37.5%) blood culture was the first or the only positive specimen.
Prolonged incubation of NR6A medium has proved to be a suitable method for detecting mycobacteremia.
评估Bactec非放射性系统在检测播散性分枝杆菌血症中的效用。
使用Bactec NR 660/HPS系统进行血培养。对来自HIV感染患者的NR6A瓶进行6周的延长培养,并每周读取一次。培养14天后,生长值≥30被认为是阳性。
从32例人类免疫缺陷病毒感染患者的80份血培养物中分离出分枝杆菌。检测到生长所需的平均时间为37.8天(范围20 - 45天)。从18例患者中分离出结核分枝杆菌,从14例患者中分离出鸟分枝杆菌。在12例患者(37.5%)中,血培养是首个或唯一的阳性标本。
NR6A培养基的延长培养已被证明是检测分枝杆菌血症的一种合适方法。