Malone M A, Costa Garcia A, Tunon Blanco P, Smyth M R
Department of Physical and Analytical Chemistry, University of Oviedo, Asturias, Spain.
J Pharm Biomed Anal. 1993 Oct;11(10):939-46. doi: 10.1016/0731-7085(93)80053-4.
Aminopterin was studied as a model compound for its analogues which maintain the pteridine ring in their structure. Its adsorptive behaviour on mercury was studied and the DC adsorptive stripping and phase-selective AC adsorptive stripping conditions were optimized. 10-Edam, an aminopterin analogue, was studied and shown to behave similarly to aminopterin. Phase-selective AC voltammetry provided the best signal and gave a detection limit of 4 x 10(-12) M aminopterin in aqueous solution employing an accumulation time of 10 min. The optimized method was applied to the analysis of both aminopterin and 10-Edam respectively in human serum. After extraction with a C18 reversed-phase cartridge the detection limit of the method was 1 x 10(-8) M aminopterin and the overall assay percentage recovery was 73.5% (n = 5) at a concentration of 5 x 10(-7) M aminopterin in serum. The analysis of 10-Edam at the same concentration in serum yielded the higher percentage recovery of 94.46% (n = 5) following the same procedure.
以氨基蝶呤作为其结构中保留蝶啶环的类似物的模型化合物进行了研究。研究了其在汞上的吸附行为，并优化了直流吸附溶出和相选择性交流吸附溶出条件。对氨基蝶呤类似物10-伊登进行了研究，结果表明其行为与氨基蝶呤相似。相选择性交流伏安法提供了最佳信号，在水溶液中采用10分钟的富集时间时，氨基蝶呤的检测限为4×10⁻¹² M。将优化后的方法分别应用于人血清中氨基蝶呤和10-伊登的分析。用C18反相柱萃取后，该方法对血清中浓度为5×10⁻⁷ M的氨基蝶呤的检测限为1×10⁻⁸ M，总分析回收率为73.5%（n = 5）。按照相同程序，对血清中相同浓度的10-伊登进行分析，回收率更高，为94.46%（n = 5）。
