Kawabe T T, Buhl A E
Biochemistry Research, Upjohn Company, Kalamazoo, Michigan 49007.
Microsc Res Tech. 1993 Dec 15;26(6):513-6. doi: 10.1002/jemt.1070260605.
We have recently modified a non-fluorescent, non-radioactive histochemical method to detect sulfhydryl (S-H) groups in tissues. This method was originally intended to detect chloramphenicol acetyltransferase (CAT) in transgenic mice. Temporal developmental differences in the keratinization of mouse digits can be seen in the staining pattern of the skin about the toes of neonatal mice. The basal cells of the epidermis exposed to the air show intense staining while the epidermis that is still attached to an adjacent toe shows no staining. The degree of S-H presence can be determined by the tissues' resistance to blocking of the S-H groups by iodoacetic acid. Areas that contain very high numbers of S-H groups still show staining following blocking by iodoacetic acid. We have found that this method shows clear differences in the S-H distribution of various epithelium, including skin, hair, nails, and tongue epithelium.
我们最近改进了一种非荧光、非放射性的组织化学方法，用于检测组织中的巯基（S-H）基团。该方法最初旨在检测转基因小鼠中的氯霉素乙酰转移酶（CAT）。在新生小鼠脚趾周围皮肤的染色模式中，可以看到小鼠趾角化的时间发育差异。暴露于空气中的表皮基底细胞显示强烈染色，而仍与相邻脚趾相连的表皮则无染色。S-H存在的程度可以通过组织对碘乙酸阻断S-H基团的抗性来确定。含有大量S-H基团的区域在被碘乙酸阻断后仍显示染色。我们发现，该方法在包括皮肤、毛发、指甲和舌上皮在内的各种上皮组织的S-H分布上显示出明显差异。
