• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

相似文献

1
PPADS selectively antagonizes P2X-purinoceptor-mediated responses in the rabbit urinary bladder.PPADS可选择性拮抗兔膀胱中P2X嘌呤受体介导的反应。
Br J Pharmacol. 1993 Dec;110(4):1491-5. doi: 10.1111/j.1476-5381.1993.tb13990.x.
2
Selective antagonism by PPADS at P2X-purinoceptors in rabbit isolated blood vessels.PPADS对兔离体血管中P2X嘌呤受体的选择性拮抗作用。
Br J Pharmacol. 1994 Mar;111(3):923-9. doi: 10.1111/j.1476-5381.1994.tb14827.x.
3
Inhibitory action of PPADS on relaxant responses to adenine nucleotides or electrical field stimulation in guinea-pig taenia coli and rat duodenum.PPADS对豚鼠结肠带和大鼠十二指肠中腺嘌呤核苷酸或电场刺激引起的舒张反应的抑制作用。
Br J Pharmacol. 1995 Aug;115(8):1509-17. doi: 10.1111/j.1476-5381.1995.tb16644.x.
4
Temperature dependency of P2 receptor-mediated responses.P2受体介导反应的温度依赖性。
Eur J Pharmacol. 2002 Dec 5;456(1-3):107-14. doi: 10.1016/s0014-2999(02)02655-9.
5
Investigation of the actions of PPADS, a novel P2x-purinoceptor antagonist, in the guinea-pig isolated vas deferens.新型P2x嘌呤受体拮抗剂PPADS对豚鼠离体输精管作用的研究。
Br J Pharmacol. 1994 Mar;111(3):913-7. doi: 10.1111/j.1476-5381.1994.tb14825.x.
6
Design and pharmacology of selective P2-purinoceptor antagonists.选择性P2嘌呤受体拮抗剂的设计与药理学
J Auton Pharmacol. 1996 Dec;16(6):341-4. doi: 10.1111/j.1474-8673.1996.tb00049.x.
7
Identification of atropine- and P2X1 receptor antagonist-resistant, neurogenic contractions of the urinary bladder.鉴定对阿托品和P2X1受体拮抗剂耐药的膀胱神经源性收缩。
J Neurosci. 2007 Jan 24;27(4):845-51. doi: 10.1523/JNEUROSCI.3115-06.2007.
8
Suramin antagonizes responses to P2-purinoceptor agonists and purinergic nerve stimulation in the guinea-pig urinary bladder and taenia coli.苏拉明可拮抗豚鼠膀胱和平滑肌对P2嘌呤受体激动剂及嘌呤能神经刺激的反应。
Br J Pharmacol. 1990 Mar;99(3):617-21. doi: 10.1111/j.1476-5381.1990.tb12979.x.
9
Prominent sympathetic purinergic vasoconstriction in the rabbit splenic artery: potentiation by 2,2'-pyridylisatogen tosylate.兔脾动脉中显著的交感嘌呤能血管收缩：对甲苯磺酸2,2'-吡啶异吲哚原的增强作用
Br J Pharmacol. 1997 Feb;120(3):530-6. doi: 10.1038/sj.bjp.0700933.
10
Assessment and characterization of purinergic contractions and relaxations in the rat urinary bladder.评估和描述大鼠膀胱的嘌呤能收缩和舒张反应。
Basic Clin Pharmacol Toxicol. 2010 Jul;107(1):603-13. doi: 10.1111/j.1742-7843.2010.00554.x. Epub 2010 Apr 12.

引用本文的文献

1
Radioligands Targeting the Purinergic P2X Receptors.靶向嘌呤能P2X受体的放射性配体
Cells. 2025 Jun 27;14(13):984. doi: 10.3390/cells14130984.
2
Pericyte pannexin1 controls cerebral capillary diameter and supports memory function.周细胞的泛连接蛋白1控制脑毛细血管直径并支持记忆功能。
Nat Commun. 2025 Jul 3;16(1):6128. doi: 10.1038/s41467-025-61312-0.
3
Purinergic inhibitory regulation of esophageal smooth muscle is mediated by P2Y receptors and ATP-dependent potassium channels in rats.嘌呤能对大鼠食管平滑肌的抑制性调节由P2Y受体和ATP依赖性钾通道介导。
J Physiol Sci. 2024 Apr 23;74(1):26. doi: 10.1186/s12576-024-00916-5.
4
The mast cell stimulator compound 48/80 causes urothelium-dependent increases in murine urinary bladder contractility.肥大细胞刺激物化合物 48/80 引起小鼠膀胱尿路上皮依赖性收缩力增加。
Am J Physiol Renal Physiol. 2023 Jul 1;325(1):F50-F60. doi: 10.1152/ajprenal.00116.2023. Epub 2023 May 18.
5
Purinergic signalling in the urinary bladder - When function becomes dysfunction.嘌呤能信号在膀胱中的作用——当功能变为功能障碍。
Auton Neurosci. 2021 Nov;235:102852. doi: 10.1016/j.autneu.2021.102852. Epub 2021 Jul 17.
6
Medicinal chemistry of P2 and adenosine receptors: Common scaffolds adapted for multiple targets.P2 和腺苷受体的药物化学：为多个靶点改编的常见支架。
Biochem Pharmacol. 2021 May;187:114311. doi: 10.1016/j.bcp.2020.114311. Epub 2020 Oct 29.
7
EFFECT OF NEW PYRIDOXAL PHOSPHATE ARYLAZO DERIVATIVES ON THE ECTO-ATPase ACTIVITY IN GUINEA PIG TISSUES.新型磷酸吡哆醛芳基偶氮衍生物对豚鼠组织中外源性ATP酶活性的影响。
Pharm Chem J. 2000 May;34(5):226-228. doi: 10.1007/BF02524624.
8
Actions of a Series of PPADS Analogs at P2X and P2X Receptors.一系列PPADS类似物对P2X和P2X受体的作用。
Drug Dev Res. 2001 Aug;53(4):281-291. doi: 10.1002/ddr.1197. Epub 2001 Oct 18.
9
Effect of type II diabetes on male rat bladder contractility.2型糖尿病对雄性大鼠膀胱收缩性的影响。
Am J Physiol Renal Physiol. 2016 May 1;310(9):F909-22. doi: 10.1152/ajprenal.00511.2015. Epub 2016 Jan 28.
10
Both RyRs and TPCs are required for NAADP-induced intracellular Ca²⁺ release.需要 RyRs 和 TPCs 来实现 NAADP 诱导的细胞内 Ca²⁺释放。
Cell Calcium. 2015 Sep;58(3):237-45. doi: 10.1016/j.ceca.2015.05.005. Epub 2015 Jun 10.

本文引用的文献

1
The Innervation of the Pelvic and adjoining Viscera: Part II. The Bladder. Part III. The External Generative Organs. Part IV. The Internal Generative Organs. Part V. Position of the Nerve Cells on the Course of the Efferent Nerve Fibres.盆腔及相邻脏器的神经支配：第二部分。膀胱。第三部分。外生殖器。第四部分。内生殖器。第五部分。传出神经纤维行程上神经细胞的位置。
J Physiol. 1895 Dec 30;19(1-2):71-139. doi: 10.1113/jphysiol.1895.sp000587.
2
Investigation of certain aspects of atropine-resistant nerve effects.阿托品抵抗性神经效应某些方面的研究
J Pharmacol Exp Ther. 1961 Feb;131:231-6.
3
The use of the slowly degradable analog, alpha, beta-methylene ATP, to produce desensitisation of the P2-purinoceptor: effect on non-adrenergic, non-cholinergic responses of the guinea-pig urinary bladder.使用缓慢降解类似物α,β-亚甲基ATP诱导P2嘌呤受体脱敏：对豚鼠膀胱非肾上腺素能、非胆碱能反应的影响
Eur J Pharmacol. 1982 Dec 24;86(2):291-4. doi: 10.1016/0014-2999(82)90330-2.
4
Ligand: a versatile computerized approach for characterization of ligand-binding systems.配体：一种用于表征配体结合系统的通用计算机化方法。
Anal Biochem. 1980 Sep 1;107(1):220-39. doi: 10.1016/0003-2697(80)90515-1.
5
A study of the atropine-resistant component of the neurogenic response of the rabbit urinary bladder.兔膀胱神经源性反应中阿托品抵抗成分的研究。
Eur J Pharmacol. 1984 Apr 6;99(4):295-302. doi: 10.1016/0014-2999(84)90136-5.
6
Purinergic receptors: photoaffinity analog of adenosine triphosphate is a specific adenosine triphosphate antagonist.嘌呤能受体：三磷酸腺苷的光亲和类似物是一种特异性三磷酸腺苷拮抗剂。
Science. 1980 Jun 13;208(4449):1273-6. doi: 10.1126/science.6103581.
7
Atropine-resistant excitatory junction potentials in rabbit bladder are blocked by alpha,beta-methylene ATP.α,β-亚甲基三磷酸腺苷可阻断兔膀胱中对阿托品耐受的兴奋性突触后电位。
Eur J Pharmacol. 1985 Aug 15;114(2):239-40. doi: 10.1016/0014-2999(85)90635-1.
8
P2-purinoceptors of two subtypes in the rabbit mesenteric artery: reactive blue 2 selectively inhibits responses mediated via the P2y-but not the P2x-purinoceptor.兔肠系膜动脉中两种亚型的P2嘌呤受体：活性蓝2选择性抑制通过P2Y嘌呤受体而非P2X嘌呤受体介导的反应。
Br J Pharmacol. 1987 Feb;90(2):383-91. doi: 10.1111/j.1476-5381.1987.tb08968.x.
9
The effect of arylazido aminopropionyl ATP (ANAPP3) on inhibition of pelvic nerve evoked contractions of the cat urinary bladder.芳基叠氮氨基丙酰ATP（ANAPP3）对猫膀胱盆神经诱发收缩的抑制作用。
Eur J Pharmacol. 1986 Jan 29;120(3):351-4. doi: 10.1016/0014-2999(86)90476-0.
10
Extracellular ATP: effects, sources and fate.细胞外ATP：作用、来源及去向
Biochem J. 1986 Jan 15;233(2):309-19. doi: 10.1042/bj2330309.

PPADS可选择性拮抗兔膀胱中P2X嘌呤受体介导的反应。

PPADS selectively antagonizes P2X-purinoceptor-mediated responses in the rabbit urinary bladder.

作者信息

Ziganshin A U, Hoyle C H, Bo X, Lambrecht G, Mutschler E, Bäumert H G, Burnstock G

机构信息

Department of Anatomy and Developmental Biology, University College London.

出版信息

Br J Pharmacol. 1993 Dec;110(4):1491-5. doi: 10.1111/j.1476-5381.1993.tb13990.x.

DOI:10.1111/j.1476-5381.1993.tb13990.x
PMID:8306091
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2175839/
Abstract
  1. Pyridoxalphosphate-6-azophenyl-2',4'-disulphonic acid (PPADS), an inhibitor of P2X-purinoceptor-mediated responses in rabbit vas deferens, was investigated for its ability to antagonize contractions evoked by alpha,beta-methylene ATP (alpha,beta-MeATP), carbachol and electrical field stimulation in the rabbit urinary bladder detrusor muscle. 2. PPADS. (1-30 microM) caused concentration-dependent inhibition of contractions to the stable P2X-purinoceptor agonist, alpha,beta-MeATP, decreasing the maximum response to alpha,beta-MeATP (30 microM) at concentrations of 3-30 microM. The pD2 value for alpha,beta-MeATP in the absence of PPADS was 6.52 +/- 0.10 (8). In the presence of PPADS at concentrations of 1, 3, 10 and 30 microM the negative log concentrations of alpha,beta-MeATP that cause the same contractile response as the pD2 value were significantly different from control, being respectively 6.17 +/- 0.09 (8), 5.64 +/- 0.12 (7), 5.15 +/- 0.23 (7) and 4.78 +/- 0.22 (5). 3. PPADS (1-30 microM) caused concentration-dependent inhibition of contractions to stimulation of intramural purinergic nerves (1-32 Hz). There was a greater inhibition at lower frequencies (1-8 Hz) than at higher frequencies (16-32 Hz). PPADS, 30 microM, did not produce significantly greater antagonism than 10 microM. 4. PPADS (30 microM) had no significant influence on the contractile potency of carbachol: the pD2 values of carbachol in the absence and presence of PPADS were not significantly different being 6.42 +/- 0.16 (5) and 6.33 +/- 0.18 (5), respectively. However, PPADS caused a small, but significant, suppression of the maximal response of carbachol, reducing it by approximately 9%. 5. Radioligand binding studies carried out on rabbit bladder membranes with [3H]-alpha,beta-methylene ATP([3H]-alpha,beta-MeATP) showed that PPADS concentration-dependently inhibited the binding of [3H]-alpha,beta-MeATP to P2X-purinoceptors, while the binding of [3H]-quinuclidinyl benzilate to muscarinic cholinoceptors was not affected.6. Thus, PPADS (1-30 microM) antagonized responses mediated via P2X-purinoceptors in the rabbit urinary bladder. It was selective for P2-purinoceptor-mediated contractions rather than those mediated via muscarinic receptors. Binding studies demonstrated that the antagonistic effect of PPADS is via a direct interaction with P2x-purinoceptors.
摘要
  1. 研究了磷酸吡哆醛-6-偶氮苯-2',4'-二磺酸（PPADS），一种兔输精管中P2X嘌呤受体介导反应的抑制剂，考察其拮抗α,β-亚甲基ATP（α,β-MeATP）、卡巴胆碱和电场刺激诱发兔膀胱逼尿肌收缩的能力。2. PPADS（1 - 30微摩尔）对稳定的P2X嘌呤受体激动剂α,β-MeATP诱发的收缩产生浓度依赖性抑制，在3 - 30微摩尔浓度下降低对α,β-MeATP（30微摩尔）的最大反应。无PPADS时α,β-MeATP的pD2值为6.52±0.10（8）。在1、3、10和30微摩尔浓度的PPADS存在下，引起与pD2值相同收缩反应的α,β-MeATP的负对数浓度与对照有显著差异，分别为6.17±0.09（8）、5.64±0.12（7）、5.15±0.23（7）和4.78±0.22（5）。3. PPADS（1 - 30微摩尔）对壁内嘌呤能神经刺激（1 - 32赫兹）诱发的收缩产生浓度依赖性抑制。低频（1 - 8赫兹）时的抑制作用大于高频（16 - 32赫兹）。30微摩尔的PPADS产生的拮抗作用并不比10微摩尔时显著更强。4. PPADS（30微摩尔）对卡巴胆碱的收缩效力无显著影响：无PPADS和有PPADS时卡巴胆碱的pD2值无显著差异，分别为6.42±0.16（5）和6.33±0.18（5）。然而，PPADS对卡巴胆碱的最大反应有轻微但显著的抑制，使其降低约9%。5. 用[3H]-α,β-亚甲基ATP（[3H]-α,β-MeATP）对兔膀胱膜进行的放射性配体结合研究表明，PPADS浓度依赖性抑制[3H]-α,β-MeATP与P2X嘌呤受体的结合，而[3H]-喹核醇基苯甲酸酯与毒蕈碱胆碱受体的结合未受影响。6. 因此，PPADS（1 - 30微摩尔）拮抗兔膀胱中通过P2X嘌呤受体介导的反应。它对P2嘌呤受体介导的收缩具有选择性，而非对通过毒蕈碱受体介导的收缩。结合研究表明，PPADS的拮抗作用是通过与P2X嘌呤受体直接相互作用实现的。