Mayatepek E, Pecher G
University Children's Hospital, University of Heidelberg, Germany.
Clin Chim Acta. 1993 Sep 30;218(2):185-92. doi: 10.1016/0009-8981(93)90182-4.
The cysteinyl leukotrienes LTC4, LTD4 and LTE4 are potent lipid mediators eliminated from the blood circulation mainly due to uptake by the liver and the kidneys. In man hepatobiliary elimination of cysteinyl leukotrienes predominates over renal excretion. In the present study, the urine from patients with extrahepatic cholestasis (n = 25) and age- and sex-matched healthy control subjects (n = 25) was analyzed for endogenous LTE4, the predominant metabolite of LTC4 excreted into urine. LTE4 was separated by reversed-phase high-performance liquid chromatography and subsequently quantified by enzyme immunoassay. Healthy subjects excreted a median concentration of 14 nmol LTE4/mol creatinine (range 5-24 nmol/mol creatinine). Its median concentration increased significantly to more than 5-fold higher levels to 74 nmol LTE4/mol creatinine (range 52-93 nmol/mol creatinine) in patients with extrahepatic cholestasis (P < 0.01). These results indicate that extrahepatic cholestasis leads to a compensatory diversion of cysteinyl leukotriene elimination to the kidney with subsequent increased excretion of LTE4 into urine.
半胱氨酰白三烯LTC4、LTD4和LTE4是强效脂质介质，主要通过肝脏和肾脏摄取而从血液循环中清除。在人类中，半胱氨酰白三烯的肝胆清除作用比肾脏排泄更为显著。在本研究中，分析了肝外胆汁淤积患者（n = 25）以及年龄和性别匹配的健康对照者（n = 25）尿液中的内源性LTE4，LTE4是排泄到尿液中的LTC4的主要代谢产物。通过反相高效液相色谱法分离LTE4，随后采用酶免疫测定法定量。健康受试者排泄的LTE4中位浓度为14 nmol/mol肌酐（范围为5 - 24 nmol/mol肌酐）。在肝外胆汁淤积患者中，其中位浓度显著增加至超过5倍，达到74 nmol LTE4/mol肌酐（范围为52 - 93 nmol/mol肌酐）（P < 0.01）。这些结果表明，肝外胆汁淤积导致半胱氨酰白三烯清除途径代偿性转向肾脏，随后尿液中LTE4排泄增加。
