Meister B, Dagerlind A, Nicholas A P, Hökfelt T
Department of Histology and Neurobiology, Karolinska Institute, Stockholm, Sweden.
Eur J Pharmacol. 1993 Nov 15;247(3):229-32. doi: 10.1016/0922-4106(93)90189-g.
Oligonucleotide probes were designed to sequences of the rat alpha 1B- and alpha 2B-adrenergic receptor mRNA and used for in situ hybridization histochemistry on tissue sections of kidneys from control and thyroidectomized rats. Both alpha 1B- and alpha 2B-receptor mRNA labelling was demonstrated in proximal tubule cells in the outer stripe of the outer medulla, with tubular rays radiating into the cortex. Thyroidectomy induced a more than 4-fold increase in mRNA for the alpha 1B-receptor in the kidney, whereas no change in alpha 2B-receptor mRNA levels could be demonstrated in thyroidectomized rats as compared to control animals. The results suggest that thyroid hormone plays an important role in regulating expression of alpha 1B-receptors in renal tubule cells.
设计了针对大鼠α1B - 和α2B - 肾上腺素能受体mRNA序列的寡核苷酸探针，并用于对对照大鼠和甲状腺切除大鼠肾脏组织切片进行原位杂交组织化学分析。在肾外髓质外层条纹的近端小管细胞中均显示出α1B - 和α2B - 受体mRNA标记，有管状射线向皮质放射。甲状腺切除术后，肾脏中α1B - 受体的mRNA增加了4倍以上，而与对照动物相比，甲状腺切除大鼠的α2B - 受体mRNA水平没有变化。结果表明，甲状腺激素在调节肾小管细胞中α1B - 受体的表达中起重要作用。
