Stimulatory action of porcine follicular fluid on granulosa cell secretion of cyclic GMP.

Effects of FSH, LH and follicular fluid isolated from preovulatory follicles on secretion of progesterone, cAMP a cGMP by cultured immature porcine granulosa cells were investigated. Exposure of granulosa cells to gonadotropins and follicular fluid resulted in increase of progesterone and cGMP formation. Follicular fluid had an inhibitory effect on cAMP synthesis. The findings suggest a possible intermediary role of cGMP for the stimulatory action of follicular fluid in the process of luteinization of granulosa cells.