Perelygina L M, Baricheva E M, Sebeleva T E, Kokoza V A
Genetika. 1993 Oct;29(10):1597-607.
A cDNA copy of the Nc70F gene which is specifically expressed in Drosophila neural tissue was cloned and characterized. The gene has an open reading frame for the protein of 384 amino acids. The protein contains dimerization, DNA binding, activation and repression domains which are common for the eucaryotic transcription factors. However, the domain organization of the Nc70F protein has some peculiarities. The primary structure of the Nc70F product and other transcription factors were compared. High level of homology of Nc70F protein with the mouse delta transcription factor was found. The in situ hybridization on tissue section showed that the Nc70F gene expression is restricted to the central nerve system at all stages of Drosophila ontogenesis. By using Drosophila genomic and cDNA clones of Nc70F genes as probes, homologous transcripts were identified in the human poly(A) +RNA. The evolutionary conservative portion of this gene was localized in the 5-exons.
克隆并鉴定了在果蝇神经组织中特异性表达的Nc70F基因的cDNA拷贝。该基因具有一个编码384个氨基酸蛋白质的开放阅读框。该蛋白质含有真核转录因子常见的二聚化、DNA结合、激活和抑制结构域。然而，Nc70F蛋白质的结构域组织有一些独特之处。比较了Nc70F产物与其他转录因子的一级结构。发现Nc70F蛋白质与小鼠δ转录因子具有高度同源性。组织切片原位杂交表明，在果蝇个体发育的所有阶段，Nc70F基因表达都局限于中枢神经系统。通过使用果蝇Nc70F基因的基因组和cDNA克隆作为探针，在人多聚腺苷酸（poly(A)+）RNA中鉴定出同源转录本。该基因的进化保守部分定位于5个外显子中。
