血液中百草枯和敌草快的提取与定量分析。

Extraction and quantitation of paraquat and diquat from blood.

作者信息

Matsuoka T, Okuda J

机构信息

Criminal Investigation Laboratory, Aichi Prefectural Police Headquarters, Nagoya, Japan.

出版信息

Forensic Sci Int. 1993 Dec;62(3):179-86. doi: 10.1016/0379-0738(93)90207-q.

DOI:10.1016/0379-0738(93)90207-q

PMID:8307528

Abstract

Trace amounts of paraquat and diquat in blood were extracted with phenol after deproteinization of blood protein with a chloroform:ethanol mixture and ammonium sulfate. The color reaction of paraquat was achieved by addition of alkaline sodium dithionite to the phenolic extract. The blue paraquat radical produced was determined directly by measurement of the absorption of the phenol layer. The assay of paraquat (> or = 0.5 micrograms) in 1.0 ml of blood (recovery, 93.4%) could be performed within 30 min. Furthermore, simultaneous analysis of paraquat and diquat in the phenolic extract of the sample could be achieved by use of second-derivative spectroscopy within 30 min.

摘要

血液中的痕量百草枯和敌草快在用氯仿

乙醇混合物和硫酸铵使血液蛋白脱蛋白后，用苯酚进行萃取。向酚提取物中加入碱性连二亚硫酸钠可实现百草枯的显色反应。所产生的蓝色百草枯自由基通过测量酚层的吸光度直接测定。在30分钟内可完成对1.0 ml血液中百草枯（≥0.5微克）的测定（回收率为93.4%）。此外，通过使用二阶导数光谱法，可在30分钟内对样品的酚提取物中的百草枯和敌草快进行同时分析。