Tsiotra P C, Karagogeos D, Theodorakis K, Michaelidis T M, Modi W S, Furley A J, Jessell T M, Papamatheakis J
Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology, Crete, Greece.
Genomics. 1993 Dec;18(3):562-7. doi: 10.1016/s0888-7543(05)80357-x.
The transient axonal glycoprotein (TAG-1) is a cell adhesion molecule that promotes neurite outgrowth and belongs to the immunoglobulin superfamily. We have isolated cDNAs encoding TAX1, the human homologue of TAG-1. Human TAX1 shows a high degree of homology to rat TAX1 and less to its chick counterpart, axonin-1, with 91 and 75% identity at the amino acid level, respectively. The numbers of immunoglobulin (IgC2) domains and fibronectin repeats present in TAG-1 are conserved among the three species. The highest degree of conservation occurs in the second IgC2 domain (98% with the rat and 82% with the chick). The human homologue also contains a putative N-terminal signal sequence and a C-terminal hydrophobic sequence, suggestive of linkage to the cell membrane via phosphatidylinositol. In addition, the two mammalian TAG-1 proteins share the RGD tripeptide, a motif known to mediate recognition of fibronectin by integrins. In situ hybridization to human metaphase chromosomes maps the TAX1 gene encoding human TAG-1 to a single location on chromosome 1q32.
瞬时轴突糖蛋白（TAG-1）是一种促进神经突生长的细胞粘附分子，属于免疫球蛋白超家族。我们已分离出编码TAX1（TAG-1的人类同源物）的cDNA。人类TAX1与大鼠TAX1高度同源，与鸡的对应物轴突素-1同源性较低，在氨基酸水平上分别具有91%和75%的同一性。TAG-1中存在的免疫球蛋白（IgC2）结构域和纤连蛋白重复序列的数量在这三个物种中是保守的。最高程度的保守发生在第二个IgC2结构域（与大鼠为98%，与鸡为82%）。人类同源物还包含一个推定的N端信号序列和一个C端疏水序列，提示通过磷脂酰肌醇与细胞膜相连。此外，两种哺乳动物的TAG-1蛋白共享RGD三肽，这是一个已知可介导整合素对纤连蛋白识别的基序。对人类中期染色体的原位杂交将编码人类TAG-1的TAX1基因定位到染色体1q32上的一个单一位置。
