Kozlov S V, Giger R J, Hasler T, Korvatska E, Schorderet D F, Sonderegger P
Institute of Biochemistry, University of Zurich, Switzerland.
Genomics. 1995 Nov 20;30(2):141-8. doi: 10.1006/geno.1995.9892.
The human TAX-1 gene (HGMW-approved symbol TAX1) is located on chromosome 1 (1q32.1) and encodes the neuronal cell adhesion molecule TAG-1/axonin-1. The gene product, termed TAG-1 in the rat and axonin-1 in the chicken, is composed of six immunoglobulin (Ig)-like and four fibronectin type III (FNIII)-like domains. It is found predominantly on the axons of particular nerve fiber tracts during neural development, and it has been demonstrated to function as a potent substratum for neurite outgrowth in vitro. Here we report the cloning and structural characterization of the TAX-1 gene. The transcribed region of the TAX-1 gene extends over about 40 kb. Like its chicken homologue, the human TAX-1 gene consists of 23 exons. Two GT/CA microsatellites were localized in the first intron; a polymorphism was found for one of them. Reporter gene analysis with serially truncated fragments of the 5'-flanking region indicated that a 164-bp fragment located immediately upstream of the putative transcription initiation site was sufficient to function as a basal promoter.
人类TAX-1基因（HGMW批准符号TAX1）位于1号染色体（1q32.1）上，编码神经元细胞粘附分子TAG-1/轴突素-1。该基因产物在大鼠中称为TAG-1，在鸡中称为轴突素-1，由六个免疫球蛋白（Ig）样结构域和四个纤连蛋白III型（FNIII）样结构域组成。在神经发育过程中，它主要存在于特定神经纤维束的轴突上，并且已证明它在体外作为神经突生长的有效底物发挥作用。在此，我们报告TAX-1基因的克隆和结构特征。TAX-1基因的转录区域延伸约40kb。与其鸡同源物一样，人类TAX-1基因由23个外显子组成。两个GT/CA微卫星位于第一个内含子中；其中一个发现了多态性。用5'侧翼区域的连续截短片段进行报告基因分析表明，位于假定转录起始位点上游紧邻的一个164bp片段足以作为基础启动子发挥作用。
