Gelb B D
Department of Pediatrics, Mount Sinai School of Medicine, New York, New York 10029.
Genomics. 1993 Dec;18(3):651-5. doi: 10.1016/s0888-7543(05)80368-4.
The mouse carnitine palmitoyltransferase II gene has been isolated and its genomic structure determined. The gene contained five exons, including an unusually long 1305-nt exon 4. The exon/intron boundaries were sequenced and conformed to the consensus splice junction sequences. The transcription start site was determined from adult mouse heart mRNA by primer extension analysis and confirmed by 5' rapid amplification of cDNA ends. A promoter that differs from the previously published promoter for the carnitine palmitoyltransferase II gene was isolated and sequenced. When placed in a mammalian expression vector, this promoter was shown to drive the chloramphenicol acetyltransferase gene in mouse hepatoma cells. This new promoter is the major driver of carnitine palmitoyltransferase II gene expression in heart and may also be active in liver.
小鼠肉碱棕榈酰转移酶II基因已被分离出来，并确定了其基因组结构。该基因包含五个外显子，其中包括一个异常长的1305个核苷酸的外显子4。对外显子/内含子边界进行了测序，结果符合共有剪接连接序列。通过引物延伸分析从成年小鼠心脏mRNA中确定了转录起始位点，并通过5' cDNA末端快速扩增进行了确认。分离并测序了一个与先前发表的肉碱棕榈酰转移酶II基因启动子不同的启动子。当置于哺乳动物表达载体中时，该启动子在小鼠肝癌细胞中可驱动氯霉素乙酰转移酶基因的表达。这个新的启动子是心脏中肉碱棕榈酰转移酶II基因表达的主要驱动因素，在肝脏中也可能具有活性。
